胶体金
生物传感器
检出限
吸附
化学
纳米颗粒
DNA
离心
色谱法
牛血清白蛋白
纳米技术
生物化学
材料科学
有机化学
作者
Yi-Ting Wang,Ruinian Hua,Juewen Liu
出处
期刊:Langmuir
[American Chemical Society]
日期:2024-09-03
卷期号:40 (37): 19839-19845
标识
DOI:10.1021/acs.langmuir.4c02763
摘要
Label-free gold nanoparticle (AuNP)-based colorimetric biosensors have been widely used for the detection of DNA. However, the effect of the biological sample matrix has not been fully explored. In this work, we investigated the salt-induced aggregation of AuNPs as well as DNA adsorption in serum and milk. AuNPs of 13, 30, and 50 nm were used as probes. The detection was successful only in a clean buffer but failed in serum or milk. Serum and milk exhibited excellent protective properties, even 250 mM NaCl added did not induce the aggregation of AuNPs. After centrifugation of milk, the supernatant did not protect the AuNPs, whereas the redispersed pellet showed protection. The limit concentration of serum to prevent AuNPs from aggregating was 0.04% for 13 nm AuNPs and 0.01% serum for 50 nm AuNPs. In addition, serum reduced DNA adsorption, and the DNA was adsorbed to the protein corona instead of directly to the AuNP surface. These two factors can explain the difficulty of detection in protein-containing samples. This study articulates the adsorption of proteins by AuNPs in biological samples and offers useful insights into the biosensor design.
科研通智能强力驱动
Strongly Powered by AbleSci AI