A sustainable bioprocess technology for producing food-flavour (+)-γ-decalactone from Castor oil-derived ricinoleic acid using enzymatic activity of Candida parapsilosis: Scale-up optimization and purification using novel composite

Ricinoleic acid (RA) from castor oil was employed in biotransformation of peach-flavoured γ-decalactone (GDL), using a Candida parapsilosis strain (MTCC13027) which was isolated from waste of pineapple crown base. Using four variables-pH, cell density, amount of RA, and temperature-the biotransformation parameters were optimized using RSM and BBD. Under optimized conditions (pH 6, 10% of microbial cells, 10g/L RA at 28°C), the conversion was maximum and resulted to 80% (+)-GDL (4.4g/L/120h) yield in shake flask (500mL). Furthermore, optimization was achieved by adjusting the aeration and agitation parameters in a 3L bioreactor, which were then replicated in a 10L bioreactor to accurately determine the amount of (+)-GDL. In bioreactor condition, 4.7g/L (>85%) of (+)-GDL is produced with 20% and 40% dissolved oxygen (1.0 vvm) at 150rpm in 72h and 66h, respectively. Further, a new Al-Mg-Ca-Si composite column-chromatography method is developed to purify enantiospecific (+)-GDL (99.9%). This (+)-GDL is 100% nature-identical as validated through