滋养层
类有机物
合胞滋养细胞
细胞生物学
胎盘
干细胞
生物
胎盘形成
胎儿
怀孕
遗传学
作者
Hao Wu,Yue Wang,Hongmei Wang
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 43-52
被引量:2
标识
DOI:10.1007/7651_2022_463
摘要
Currently, human trophoblast stem cell (hTSC) is considered to be the most promising laboratory model stimulating trophoblast criteria. Our group has established hTSCs allowing differentiation to syncytiotrophoblast (STB) and extravillous trophoblast (EVT). Further, hTSC-based three-dimensional (3D) trophoblast organoid (hTSC-organoid) provides a transformative model for studying human placental development and the interaction between trophoblast and maternal environment. Here, we present a protocol to obtain different types of placental trophoblast cells and trophoblast organoids using hTSCs. The generation of hTSC-organoids takes 6 days. hTSC-organoids permit passaging and can differentiate into EVT lineage.
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