In-vitro study on chitosan/PVA incorporated with nickel oxide nanoparticles for wound healing application

Wound healing is a predominant problem in the clinical field, since it remedies the severe damage due to bacterial infections in the wound site. Nanomaterials are utilized to accelerate wound healing through their antibacterial property and cell proliferation. In this research work, the nickel oxide nanoparticles (NiO NPs) were prepared by the sol-gel method. The physicochemical properties of the synthesized nanoparticles were investigated. Then, the prepared NiO NPs blended with Chitosan (CS) and polyvinyl alcohol (PVA) film by the solution casting technique. The prepared CS/PVA/NiO films were subjected to XRD, FTIR and SEM analysis to elucidate the structural, functional, and morphological properties. Further, the contact angle, tensile strength, swelling and degradation of the prepared films were examined. The in-vitro wound healing properties of CS/PVA/NiO films exhibited better hemocompatibility and excellent antibacterial activity against wound pathogens. Cytotoxicity and migration assay shows significant wound healing properties. The obtained results suggested that the prepared CS/PVA/NiO films would be a potential candidate for the wound dressing application.