蛋白质组
蛋白质组
污渍
牙菌斑
生物
微生物学
蛋白质组学
染色
生物化学
遗传学
基因
作者
Christophe Hirtz,Atef Mannaa,Estelle Moulis,Olivier Pible,Robin O’flynn,Jean Armengaud,Virginie Jouffret,Camille Lemaistre,Gabriel Dominici,Alex Yahiaoui Martinez,Catherine Dunyach-Rémy,Laurent Tiers,Jean‐Philippe Lavigne,Paul Tramini,Marie-Christine Goldsmith,Sylvain Lehmann,Dominique Deville de Périère,Jérôme Vialaret
出处
期刊:ACS omega
[American Chemical Society]
日期:2022-02-25
卷期号:7 (10): 8258-8267
被引量:6
标识
DOI:10.1021/acsomega.1c04770
摘要
The present study focuses on the use of a metaproteomic approach to analyze Black Extrinsic Tooth Stains, a specific type of pigmented extrinsic substance. Metaproteomics is a powerful emerging technology that successfully enabled human protein and bacterial identification of this specific dental biofilm using high-resolution tandem mass spectrometry. A total of 1600 bacterial proteins were identified in black stain (BS) samples and 2058 proteins in dental plaque (DP) samples, whereas 607 and 582 human proteins were identified in BS and DP samples, respectively. A large diversity of bacteria genera (142) in BS and DP was identified, showing a high prevalence of Rothia, Kingella, Neisseria, and Pseudopropionibacterium in black stain samples. In this work, the high diversity of the dental microbiota and its proteome is highlighted, including significant differences between black stain and dental plaque samples.
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