Chemical Profile by Gas Chromatography/Mass Spectrometry of Ethyl Acetate and N-butanol Extracts of Centaurea tougourensis Boiss. & Reut

Nowadays, plants bioactive compounds are considered as a new source of therapy, especially for the elaboration process of more effective drugs. 80% of the actual drug substances are purely natural and originate from plants, representing a new hope, especially for the treatment of chronic illnesses. The aim of this study is to characterize the phytochemical composition of C. tougourensis via gas chromatography-mass spectrometry (GC-MS) approach. This method allowed the identification of 45 compounds in the n-butanol extract (n-BuOH), in which 12 compounds were in majority, namely; 2H-Furo [2,3-b] indole, 3,3a,8,8atetrahydro-2,3-dimethyl- (16.12%), Benz [c] pyran-1,3-dione, 4,4-dimethyl- (14.87%), Octadecanoic acid,9-oxo-, methyl ester (9.69%), Hydromorphone (7.94%), Acetamide, N-[3-[2-(2,3-dihydro-3-hydroxy-2-oxo-3-indolyl)-1-oxoethyl]p (6.14%), 2,6-Di-n-propyl-4-(2-furyl)pyridine (6.11%), Norhydrocodone (5.98%), Anthracene, 1,2,3,4,5,6,7,8-octahydro- (4.72%), 2,4,6-Trimethylbenzonitrile, N-oxide (4.47%), 3-O-Methyl-d-glucose (2.94%), 3,5-Diethyl-4-(2-furyl) pyridine (2.75%) and 3H-Pyrrolo [3,2-f] quinoline, 5-methoxy-1,2,7,9-tetramethyl- (2.30%). Concerning the ethyl acetate (EA) extract; 23 compounds were identified, in which 13 compounds were in majority, namely; [Bi-1,4-cyclohexadien-1-yl]-3,3,′6,6′-tetrone, 4,4′-dihydroxy-2,2,′5,5′-tetramethyl- (27.31%), Thiosulfuric acid, S-(2-aminoethyl) ester (15.07%), 2-Pentadecanone, 6,10,14-trimethyl- (8.91%), 3-Methyl-4-(phenylthio)-2-prop-2-enyl-2,5-dihydrothiophene 1,1-dioxide (7.12%), Tetrapentacontane, 1,54-dibromo- (5.74%), Heptacos-1-ene (5.03%), Propionic acid, 3-iodo-, tetradecyl ester (4.92%), 2-Methyl-E-7-octadecene (4.68%), 7,8-Epoxylanostan-11-ol, 3-acetoxy- (3.65%), 4-Fluoro-1-methyl-5-carboxylic acid, ethyl(ester) (3.15%), Tetrapentacontane, 1,54-dibromo- (2.79%), Undec-10-ynoic acid, tetradecyl ester (2.77%), 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (2.72%).