再生障碍性贫血
祖细胞
造血
人类白细胞抗原
免疫学
生物
抗原
CD8型
干细胞
骨髓
细胞生物学
作者
Noriaki Tsuji,Kohei Hosokawa,Ryota Urushihara,Mikoto Tanabe,Yoshitaka Zaimoku,Takamasa Katagiri,Tatsuhiko Ozawa,Hiroyuki Takamatsu,Ken Ishiyama,Hirohito Yamazaki,Hiroyuki Kishi,Seishi Ogawa,Shinji Nakao
出处
期刊:Leukemia
[Springer Nature]
日期:2022-04-26
卷期号:36 (6): 1666-1675
被引量:3
标识
DOI:10.1038/s41375-022-01549-6
摘要
To determine whether antigen presentation by HLA-DR on hematopoietic stem progenitor cells (HSPCs) is involved in the development of acquired aplastic anemia (AA), we studied the HLA-DR expression on CD45dimCD34+CD38+ cells in the peripheral blood of 61 AA patients including 23 patients possessing HLA-class I allele-lacking (HLA-class I[−]) leukocytes. HLA-DR-lacking (DR[−]) cells accounted for 13.0–57.1% of the total HSPCs in seven (11.5%) patients with HLA-DR15 who did not possess HLA-class I(–) leukocytes. The incubation of sorted DR(–) HSPCs in the presence of IFN-γ for 72 h resulted in the full restoration of the DR expression. A comparison of the transcriptome profile between DR(–) and DR(+) HSPCs revealed the lower expression of immune response-related genes including co-stimulatory molecules (e.g., CD48, CD74, and CD86) in DR(–) cells, which was not evident in HLA-class I(–) HSPCs. DR(–) cells were exclusively detected in GPI(+) HSPCs in four patients whose HSPCs could be analyzed separately for GPI(+) and GPI(–) HSPCs. These findings suggest that CD4+ T cells specific to antigens presented by HLA-DR15 on HSPCs may contribute to the development of AA as well as the immune escape of GPI(–) HSPCs in a distinct way from CD8+ T cells recognizing HLA-class I-restricted antigens.
科研通智能强力驱动
Strongly Powered by AbleSci AI