Endonuclease-driven DNA walking for constructing a novel colorimetric and electrochemical dual-mode biosensing method

化学 生物传感器 检出限 DNA 核酸内切酶 脱氧核酶 血红素 适体 底漆(化妆品) 纳米技术 生物物理学 色谱法 生物化学 分子生物学 材料科学 生物 血红素 有机化学
作者
Xiaojun Wang,Tingting Xuan,Wan Huang,Xin Li,Guosong Lai
出处
期刊:Analytica Chimica Acta [Elsevier BV]
卷期号:1208: 339835-339835 被引量:7
标识
DOI:10.1016/j.aca.2022.339835
摘要

The development of methods to realize the on-site analysis of antibiotic pollutants is of great importance for food quality control and environmental monitoring. Herein, we designed a magnetic bead (MB)-based DNA walker and utilized its target-triggered and endonuclease-driven walking reaction to develop a novel colorimetric and electrochemical dual-mode biosensing method for the convenient detection of kanamycin (Kana) antibiotic. The colorimetric signal transduction strategy of the method was constructed on the telomerase extension of the DNA walking-released telomeric primer into G-quadruplex/hemin DNAzymes. Due to the DNA walking and telomerase dual signal amplification, a good linear relationship from 0.1 pg mL-1 to 1 ng mL-1 was obtained for this strategy with a detection limit of 22 fg mL-1. Meanwhile, the MB complex produced through the above DNA walking reaction was also used as a multipedal DNA walker to develop an electrochemical signal transduction strategy. By utilizing it to trigger another endonuclease-driven DNA walking at a DNA hairpin-modified electrode, ferrocene labels were quantitatively released from this electrode to cause the electrochemical signal decrease. Because of the dual endonuclease-driven DNA walking for signal amplification, a five-order of magnitude wide linear relationship from 0.01 pg mL-1 to 1 ng mL-1 was obtained with an ultralow detection limit of 8.4 fg mL-1. As the two strategies did not involve complicated manipulations and the requirement of expensive instruments, this biosensing method exhibits a high application value for the on-site semiquantitative screening and accurate analysis of antibiotic residues.
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