亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Environmental relevant concentrations of triclosan affected developmental toxicity, oxidative stress, and apoptosis in zebrafish embryos

氧化应激 斑马鱼 毒性 细胞凋亡 生物 发育毒性 吖啶橙 半胱氨酸蛋白酶3 分子生物学 三氯生 活性氧 化学 男科 生物化学 基因 程序性细胞死亡 遗传学 有机化学 病理 医学 妊娠期 怀孕
作者
Fei Liu,Ying Zhang,Fan Wang
出处
期刊:Environmental Toxicology [Wiley]
卷期号:37 (4): 848-857 被引量:35
标识
DOI:10.1002/tox.23448
摘要

Triclosan (TCS), as a broad-spectrum antibacterial agent, is widely used in various pharmaceutical and personal care products. However, the details of ecological environmental health risks of TCS are not clear. In this study, zebrafish embryos/larval were exposed to environmentally relevant concentrations of TCS to evaluate the developmental toxicity. Four-hour post-fertilization (hpf) zebrafish embryos were exposed to 0, 2, 10, 50, and 250 μg/L TCS until 96 h. The heart beats at 72 hpf were significantly increased in 2 μg/L TCS group, while significantly decreased in 250 μg/L TCS treated group compared with control. The results of acridine orange staining, terminal deoxynucleotide transferase-mediated UTPnick end labeling assay, and detection of mitochondrial membrane potential showed that 50 and 250 μg/L TCS resulted in apoptosis. Meanwhile, reactive oxygen species (ROS) and DNA damage were induced, but SOD activity was significantly decreased in 250 μg/L TCS treated group. In addition, SOD(Mn) and GPx gene mRNA expressions were significantly down-regulated in 50 and 250 μg/L TCS treated groups, while Casp3, Casp9, Puma, Casp8, Apaf1, and Bid genes in 250 μg/L TCS and Mdm2 gene in 50 μg/L treated groups were significantly up-regulated. P53 protein was significantly up-regulated in 250 μg/L TCS treated group. The overall results showed that TCS can cause oxidative stress and result in apoptosis via the involvement of ROS-p53-caspase-dependent apoptotic pathway in zebrafish embryos. The present findings suggest the potential mechanisms of TCS-induced developmental toxicity appears to be the generation of ROS and the consequent triggering of apoptosis genes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
英姑应助核桃采纳,获得10
24秒前
科研通AI5应助核桃采纳,获得10
24秒前
科研通AI5应助核桃采纳,获得10
24秒前
可爱的函函应助核桃采纳,获得10
24秒前
Liufgui应助核桃采纳,获得10
25秒前
在水一方应助核桃采纳,获得10
25秒前
隐形曼青应助科研通管家采纳,获得10
46秒前
丘比特应助科研通管家采纳,获得10
46秒前
爆米花应助科研通管家采纳,获得10
46秒前
LONG完成签到 ,获得积分10
58秒前
秋风今是完成签到 ,获得积分10
1分钟前
1分钟前
核桃发布了新的文献求助10
1分钟前
biubiubiu驳回了852应助
1分钟前
AUGKING27完成签到 ,获得积分10
2分钟前
2分钟前
2分钟前
2分钟前
无语的诗柳完成签到 ,获得积分10
2分钟前
nina完成签到 ,获得积分10
2分钟前
远山淡影_cy完成签到,获得积分20
2分钟前
康谨完成签到 ,获得积分10
2分钟前
blenx完成签到,获得积分10
2分钟前
2分钟前
小叶子完成签到 ,获得积分10
2分钟前
Liufgui应助徐悦月采纳,获得10
2分钟前
2分钟前
Dave完成签到,获得积分10
3分钟前
FashionBoy应助Dave采纳,获得10
3分钟前
3分钟前
3分钟前
3分钟前
顾矜应助Emon采纳,获得10
3分钟前
3分钟前
4分钟前
芝麻完成签到,获得积分10
4分钟前
4分钟前
Dave发布了新的文献求助10
4分钟前
大模型应助芝麻采纳,获得10
4分钟前
4分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990045
求助须知:如何正确求助?哪些是违规求助? 3532108
关于积分的说明 11256334
捐赠科研通 3270943
什么是DOI,文献DOI怎么找? 1805146
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228