生物相容性
明胶
材料科学
纳米技术
药物输送
喷墨打印
喷嘴
微流控
粘度
化学
生物加工
组织工程
生物医学工程
复合材料
墨水池
机械工程
冶金
工程类
医学
生物化学
作者
Mingjun Xie,Qing Gao,Haiming Zhao,Jing Nie,Zhenliang Fu,Haoxuan Wang,Lulu Chen,Lei Shao,Jianzhong Fu,Zichen Chen,Yong He
出处
期刊:Small
[Wiley]
日期:2018-12-20
卷期号:15 (4)
被引量:109
标识
DOI:10.1002/smll.201804216
摘要
Abstract Low‐concentration gelatin methacryloyl (GelMA) has excellent biocompatibility to cell‐laden structures. However, it is still a big challenge to stably fabricate organoids (even microdroplets) using this material due to its extremely low viscosity. Here, a promising electro‐assisted bioprinting method is developed, which can print low‐concentration pure GelMA microdroplets with low cost, low cell damage, and high efficiency. With the help of electrostatic attraction, uniform GelMA microdroplets measuring about 100 μm are rapidly printed. Due to the application of lower external forces to separate the droplets, cell damage during printing is negligible, which often happens in piezoelectric or thermal inkjet bioprinting. Different printing states and effects of printing parameters (voltages, gas pressure, nozzle size, etc.) on microdroplet diameter are also investigated. The fundamental properties of low‐concentration GelMA microspheres are subsequently studied. The results show that the printed microspheres with 5% w/v GelMA can provide a suitable microenvironment for laden bone marrow stem cells. Finally, it is demonstrated that the printed microdroplets can be used in building microspheroidal organoids, in drug controlled release, and in 3D bioprinting as biobricks. This method shows great potential use in cell therapy, drug delivery, and organoid building.
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