转染
基因
异源表达
细胞培养
基因表达
生物
细胞生物学
功能(生物学)
选择(遗传算法)
体外
计算生物学
异源的
HEK 293细胞
遗传学
重组DNA
计算机科学
人工智能
作者
Richard M. Mortensen,Robert E. Kingston
标识
DOI:10.1002/0471142727.mb0905s86
摘要
To determine the function of a gene in vitro, expression in heterologous cells is often employed. This can be done by transient expression, but often requires a more permanent expression of the gene and the creation of a cell line. This process can involve decisions as to the nature of construct used for expression, and invariably uses some strategy to select the transfected cells. Typically, these strategies use one of a number of genes that confer resistance to an added drug that will kill untransfected cells but not the transfected cells (positive selection). Alternatively, sometimes the strategy uses a gene that will confer sensitivity to a compound and kills the transfected cells (negative selection). This chapter discusses some of the strategies and genes used in creating cell line for in vitro study of gene function.
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