菁
光热治疗
材料科学
荧光
赫拉
光化学
细胞毒性
猝灭(荧光)
生物物理学
化学
纳米技术
体外
光学
生物化学
生物
物理
作者
Xiujie Zhao,Zhiwen Fan,Yanqi Qiao,Yun Chen,Shuo Wang,Xinmin Yue,Tangliang Shen,Wenting Liu,Jie Yang,Gao Hong,Xuelin Zhan,Luqing Shang,Yongmei Yin,Wei Zhao,Dan Ding,Rimo Xi,Meng Meng
标识
DOI:10.1021/acsami.0c01715
摘要
Near-infrared (NIR) fluorescent probes can deeply penetrate through tissues with little damage. To facilitate image-guided theranostics, researchers usually apply a desired amount of photosensitizers to achieve effective photothermal responses. However, these probes could easily suffer from low photostability and aggregated-caused quenching effect in high concentrations. In this paper, the rational incorporation of an aggregated-induced emission (AIE) unit into the structure of heptamethine cyanine IR-780 is reported. Using tetraphenylethene (TPE) as an AIE core, we synthesize three TPE-modified IR-780 probes (IR-780 AIEgens) via different linkages. The IR-780 derivatives all show enhanced AIE features, in which the probe with an ether linkage (IR780-O-TPE) is superior in rapid cell uptake, high targeting capacity, and good photostability. Moreover, IR780-O-TPE exhibits the strongest cytotoxicity to HeLa cells (IC50 = 3.3 μM). The three IR-780 derivatives displayed a photothermal response in a concentration-dependent manner, in which IR-780 AIEgens are more cytotoxic than IR-780, with IC50 of 0.3 μM under 808 nm laser irradiation. In tumor-bearing mice, the optimal probe IR780-O-TPE also showed a more effective photothermal response than IR-780. By illustrating the relationship between aggregation state with photophysical properties, cell imaging, and cytotoxicity, this work is helpful in modulating NIR-based photosensitizers into AIE features for efficient image-guided theranostics.
科研通智能强力驱动
Strongly Powered by AbleSci AI