Linking genes of unknown function with abiotic stress responses by high‐throughput phenotype screening

拟南芥 生物 非生物胁迫 基因 非生物成分 蛋白质组 突变体 表型 拟南芥 遗传学 功能(生物学) 细胞生物学 生态学
作者
Liang Song,Alicia Hegie,Nobuhiro Suzuki,Elena Shulaev,Xiaozhong Luo,Diana Cenariu,Vincent Ma,Stephanie Kao,Jennie Lim,Meryem Betul Gunay,Teruko Oosumi,Seung Cho Lee,Jeffery F. Harper,John C. Cushman,Martin Gollery,Thomas Girke,Julia Bailey‐Serres,Rebecca A. Stevenson,Jian‐Kang Zhu,Ron Mittler
出处
期刊:Physiologia Plantarum [Wiley]
卷期号:148 (3): 322-333 被引量:102
标识
DOI:10.1111/ppl.12013
摘要

Over 13% of all genes in the Arabidopsis thaliana genome encode for proteins classified as having a completely unknown function, with the function of >30% of the Arabidopsis proteome poorly characterized. Although empirical data in the form of mRNA and proteome profiling experiments suggest that many of these proteins play an important role in different biological processes, their functional characterization remains one of the major challenges in modern biology. To expand the annotation of genes with unknown function involved in the response of Arabidopsis to different environmental stress conditions, we selected 1007 such genes and tested the response of their corresponding homozygous T-DNA insertional mutants to salinity, oxidative, osmotic, heat, cold and hypoxia stresses. Depending on the specific abiotic stresses tested, 12-31% of mutants had an altered stress-response phenotype. Interestingly, 832 out of 1007 mutants showed tolerance or sensitivity to more than one abiotic stress treatment, suggesting that genes of unknown function could play an important role in abiotic stress-response signaling, or general acclimation mechanisms. Further analysis of multiple stress-response phenotypes within different populations of mutants revealed interesting links between acclimation to heat, cold and oxidative stresses, as well as between sensitivity to ABA, osmotic, salinity, oxidative and hypoxia stresses. Our findings provide a significant contribution to the biological characterization of genes with unknown function in Arabidopsis and demonstrate that many of these genes play a key role in the response of plants to abiotic stresses.
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