Characterization of the products generated from oversulphated dermatan sulphate isomers with chondroitinase-B by high-performance liquid chromatography

Characterization with specific chondroitinase-B of the constituents of the unsaturated sulphated disaccharides generated from variously sulphated dermatan sulphate (DS) isomers in human kidney tissues was carried out by high-performance liquid chromatography, using a sulphonized styrene-divinylbenzene copolymer. The products derived from the variously sulphated DS isomers with the chondroitinase-B at specific unsaturated disaccharides were characterized as unsaturated mono-, di- and trisulphated disaccharides. The results suggest that chondroitinase-B converts DS isomers into unsaturated 4-sulphated disaccharide, unsaturated disulphated disaccharides types B and H (ΔDi-diS B and ΔDi-diS H ) and unsaturated trisulphated disaccharide (Δi-triS), which possess sulphate linkage (s) at position 4 of the galactosamine residues and, in addition, at position 6 of galactosamine and/or position 2 of iduronic acid. The ΔDi-diS B , ΔDi-diS H and ΔDi-triS were found for the first time in human kidney tissue.