The Retention Behavior of Selected Porphyrins on Silica Gel, Polyamide, and Cellulose TLC Plates

The chromatographic behavior of uroporphyrin I, uroporphyrin III, coproporphyrin I, coproporphyrin III, and protoporphyrin IX has been examined using silica gel 60F 254 , polyamide 11F 254 , and cellulose F TLC plates as stationary phases. Homologous series of n -alcohols, acetonitrile, and tetrahydrofuran were used as mobile phases. Chromatographic retention data and a possible retention mechanism are discussed. The electrostatic interactions operating in these chromatographic systems were analyzed by studying relationship between log (1/ R F ) values and the molecular polarizabilities of the solvents.