结直肠癌
DNA甲基化
生物
甲基化
癌症研究
运动性
癌症
转移
表观遗传学
病理
基因表达
DNA
医学
基因
细胞生物学
遗传学
作者
Hanguang Hu,Dehao Wu,Xibo Liu,Haifeng Yu,Junxi Xu,Wen Cai,Yanqin Huang,Rui Bai,Jiawei Zhang,Ying Gu,Shu Zheng,Weiting Ge
出处
期刊:Epigenomics
[Future Medicine]
日期:2021-08-01
卷期号:13 (16): 1269-1282
被引量:2
标识
DOI:10.2217/epi-2021-0231
摘要
Aim: The authors previously found that SPARCL1 functions to suppress colorectal cancer metastasis. Here, the epigenetic mechanism of SPARCL1 regulation and its relationship with clinicopathological features in colon cancer were investigated. Materials & methods: SPARCL1 expression was evaluated by immunohistochemistry staining in a tissue array containing 271 left-sided colon cancer samples and 257 right-sided colon cancer samples. In vivo and in vitro DNA methylation states were measured by biochemical sulfide potential assay. The transcription and DNA methylation states in cells were altered by siRNA or decitabine treatment, respectively. Cellular motility properties were compared through transwell assay. Results & conclusion: SPARCL1, mediated by its DNA methylation, may arrest colorectal carcinoma motility. Furthermore, SPARCL1 expression is higher and may have a specific prognostic value in left-sided colon cancer.
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