Improvement in Angiogenesis and Restoration of Blood Flow in Diabetic Mice by Sanguinate TM

医学 结扎 缺血 血管生成 血流 链脲佐菌素 新生血管 糖尿病 激光多普勒测速 股动脉 后肢 微血管 腹腔注射 治疗性血管生成 川地31 内科学 内分泌学 外科
作者
Xiaoping Shen,Rosa Rosario,Yu Zou,Ann Marie Schmidt,Abraham Abuchowski,Shi‐Fang Yan
出处
期刊:The FASEB Journal [Wiley]
卷期号:25 (S1) 被引量:1
标识
DOI:10.1096/fasebj.25.1_supplement.1091.4
摘要

To improve angiogenesis and restoration of blood flow in diabetic mice subjected to hind limb ischemia, here we test the hypothesis that Sanguinate (PEG-COHb), which resides in the plasma and increases the efficiency of oxygen transfer, promotes these improvements. Wild type C57BL/6 mice at age 6 weeks were rendered diabetic with streptozotocin (stz) and at age 12 weeks were subjected to femoral artery (FA) ligation followed by administration of PEG-COHb (20 ml/kg/day) via intraperitoneal (IP) injection daily for 4 weeks. Vehicle treated diabetic mice received IP injection with equal volumes of 1xPBS daily. Diabetic mice treated with PEG-COHb displayed significantly improved blood flow ratios (ischemia/sham) by laser Doppler analysis on day 28 after FA ligation compared to vehicle treated group (63.36 ± 5.83 vs 44.39 ± 4.13 BFR (%), n≥9, p=0.019). Immunohistochemistry with anti-CD31 IgG demonstrated significantly increased microvessel density in the muscle tissue of diabetic mice treated with SanguinateTM on day 28 after FA ligation compared to vehicle treated group (663.86 ± 57.78 vs 461.44 ± 36.19 capillaries/mm2, p<0.0001). Taken together, these data reveal significant therapeutic effects of SanguinateTM in diabetic limb ischemia in a murine model and may provide the basis for the use of this agent as complementary vascular-salvaging therapy in long-standing diabetes.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
1秒前
2秒前
2秒前
3秒前
3秒前
lm发布了新的文献求助10
4秒前
4秒前
4秒前
zhao完成签到,获得积分10
4秒前
weiwei完成签到,获得积分10
4秒前
科研通AI2S应助姜凯采纳,获得10
5秒前
孙福禄应助西西采纳,获得10
6秒前
孙福禄应助西西采纳,获得10
6秒前
6秒前
单纯青槐发布了新的文献求助10
6秒前
6秒前
6秒前
luckbaby完成签到,获得积分10
7秒前
方赫然发布了新的文献求助10
7秒前
JerryZ发布了新的文献求助10
7秒前
今后应助高贵绿真采纳,获得10
7秒前
Ava应助mariawang采纳,获得10
7秒前
7秒前
菜不透发布了新的文献求助10
8秒前
8秒前
纸猫发布了新的文献求助10
8秒前
9秒前
weiwei发布了新的文献求助10
9秒前
poli发布了新的文献求助10
10秒前
诚心尔琴发布了新的文献求助10
11秒前
11秒前
fgh完成签到,获得积分20
11秒前
CipherSage应助科研通管家采纳,获得10
12秒前
CAOHOU应助科研通管家采纳,获得10
12秒前
NexusExplorer应助科研通管家采纳,获得10
12秒前
SYLH应助科研通管家采纳,获得10
12秒前
英俊的铭应助科研通管家采纳,获得10
12秒前
Akim应助科研通管家采纳,获得10
12秒前
SYLH应助科研通管家采纳,获得10
12秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988786
求助须知:如何正确求助?哪些是违规求助? 3531116
关于积分的说明 11252493
捐赠科研通 3269766
什么是DOI,文献DOI怎么找? 1804771
邀请新用户注册赠送积分活动 881870
科研通“疑难数据库(出版商)”最低求助积分说明 809021