鞘磷脂
化学
膜
脂质双层
磷脂酰胆碱
膜脂
磷脂
脂筏
癌细胞
生物化学
生物物理学
小泡
细胞膜
膜流动性
生物
癌症
遗传学
作者
James C. S. Ho,Sartaj Ahmad Mir,Michele Cavalera,Parisa Esmaeili,Tuan Tran,Zandra Chew Yann,Tran Thi Hien,Arunima Chaudhuri,Anne K. Bendt,Markus R. Wenk,Catharina Svanborg
出处
期刊:Biofactors
[Wiley]
日期:2022-04-07
卷期号:48 (5): 1145-1159
被引量:1
摘要
Complexes formed by the alpha1 N-terminal peptide of alpha-lactalbumin and oleic acid (alpha1-oleate) interact with lipid bilayers. Plasma membrane perturbations trigger tumor cell death but normal differentiated cells are more resistant, and their plasma membranes are less strongly affected. This study examined membrane lipid composition as a determinant of tumor cell reactivity. Bladder cancer tissue showed a higher abundance of unsaturated lipids enriched in phosphatidylcholine, PC (36:4) and PC (38:4), and sphingomyelin, SM (36:1) than healthy bladder tissue, where saturated lipids predominated and the lipid extracts from bladder cancer tissue inhibited the tumoricidal effect of the complex more effectively than healthy tissue extracts. Furthermore, unsaturated PC in solution inhibited tumor cell death, and the complex interacted with giant unilamellar vesicles formed by PC, confirming the affinity of alpha1-oleate for fluid membranes enriched in PC. Quartz Crystal Microbalance with dissipation monitoring (QCM-D) detected a preference of the complex for the liquid-disordered phase, suggesting that the insertion into PC-based membranes and the resulting membrane perturbations are influenced by membrane lipid saturation. The results suggest that the membrane lipid composition is functionally important and that specific unsaturated membrane lipids may serve as "recognition motifs" for broad-spectrum tumoricidal molecules such as alpha1-oleate.
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