亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Angiotensin (ang) 1-7 inhibits ang II-induced atrial fibrosis through regulating the interaction of proto-oncogene tyrosine-protein kinase Src (c-Src) and Src homology region 2 domain-containing phosphatase-1 (SHP-1))

血管紧张素II CTGF公司 原癌基因酪氨酸蛋白激酶Src 时间1 内分泌学 内科学 化学 生长因子 纤维化 酪氨酸激酶 心肌纤维化 罗亚
作者
Li Li,Li Cao,Yihao Liu,Yunlin Chen,Jinqi Fan,Yuehui Yin
出处
期刊:Bioengineered [Taylor & Francis]
卷期号:12 (2): 10823-10836
标识
DOI:10.1080/21655979.2021.1967035
摘要

To verify whether Ang-(1-7) produces an antagonistic effect on Ang II-mediated atrial remodeling. Ang II-induced HL-1 cell model and a rat model of Ang II-induced atrial remodeling were constructed and intervened with Ang II Ang-(1-7), AngII +Ang-(1-7), Ang II+ c-Src specific inhibitor (SU6656), and Ang II + Ang-(1-7) + SSG (SHP-1/2 specific inhibitor, stibogluconate), respectively. The systolic blood pressure of the rat caudal artery was detected. And trial fibrosis was detected by Picrosirius red staining and Masson's trichrome staining. Expressions of transforming growth factor-β (TGF-β), tissue inhibitor of metalloproteinases 1 (TIMP1), Matrix metalloproteinase 2 (MMP-2), connective tissue growth factor (CTGF), galectin-3, α-smooth muscle actin (α-SMA), and collagen I/III were subjected to qPCR and western blot. Furthermore, SHP-1 binding to c-Src was verified by co-immunoprecipitation (Co-IP). Results showed that the expressions of TGF-β, TIMP1, MMP-2, CTGF, α-SMA, galectin-3, and collagen I were increased markedly in the Ang II intervention group, and the expressions of p-ERK1/2, p-Akt, and p-p38MAPK were also increased dramatically. Ang-(1-7) or SU6656 addition could inhibit the action of Ang II factor, thereby minimizing the expressions of the previously described genes and proteins. Simultaneously, SSG supplement reversed the antagonistic effect of Ang-(1-7) on Ang II, and the latter elevated the blood pressure and induced atrial fibrosis in rats. Ang-(1-7) could reverse the changes related to Ang II-induced atrial fibrosis in rats. In conclusion, Ang-(1-7) antagonized Ang II-induced atrial remodeling by regulating SHP-1 and c-Src, thereby affecting the MAPKs/Akt signaling pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
身法马可波罗完成签到 ,获得积分10
7秒前
9秒前
子凡完成签到 ,获得积分10
9秒前
twrw完成签到,获得积分20
11秒前
虞美人发布了新的文献求助10
17秒前
科研通AI5应助XWY采纳,获得10
21秒前
顾矜应助罗皮特采纳,获得10
23秒前
猫猫逃离二次元完成签到,获得积分10
23秒前
24秒前
28秒前
大意的绿蓉完成签到,获得积分10
28秒前
29秒前
32秒前
尊敬雪萍发布了新的文献求助10
34秒前
英姑应助重要的夏烟采纳,获得10
36秒前
李健应助ssynkl采纳,获得10
36秒前
Leffzeng完成签到,获得积分10
37秒前
demonox发布了新的文献求助10
38秒前
XWY完成签到,获得积分10
39秒前
Ava应助Leffzeng采纳,获得10
43秒前
XWY发布了新的文献求助10
45秒前
qiu发布了新的文献求助10
52秒前
ehsl完成签到,获得积分10
53秒前
李清湛完成签到,获得积分10
1分钟前
1分钟前
1分钟前
Leffzeng发布了新的文献求助10
1分钟前
随便发布了新的文献求助10
1分钟前
青山完成签到 ,获得积分10
1分钟前
1分钟前
从容成危完成签到,获得积分10
1分钟前
会飞的鱼发布了新的文献求助10
1分钟前
冰冰完成签到,获得积分20
1分钟前
1分钟前
阿司匹林完成签到 ,获得积分10
1分钟前
Ying完成签到,获得积分10
1分钟前
yuwen发布了新的文献求助10
1分钟前
QiongYin_123完成签到 ,获得积分10
1分钟前
苏梨子完成签到,获得积分10
1分钟前
余念安完成签到 ,获得积分10
1分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968293
求助须知:如何正确求助?哪些是违规求助? 3513229
关于积分的说明 11166833
捐赠科研通 3248478
什么是DOI,文献DOI怎么找? 1794268
邀请新用户注册赠送积分活动 874956
科研通“疑难数据库(出版商)”最低求助积分说明 804629