Preparation of Assemblable Chondral and Subchondral Bone Microtissues for Osteochondral Tissue Engineering

Microtissues exhibit great advantages in injecting with minimum invasiveness, mimicking natural tissues, and promoting tissue regeneration. However, very few studies have focused on the construction of osteochondral microtissues that could simultaneously support hyaline-like cartilage and bone tissue regeneration. In this study, chondral microtissues that could favor the formation of hyaline-like cartilages and subchondral bone microtissues that could repair subchondral defects to support the neo-generated cartilages were successfully constructed for osteochondral tissue engineering. For chondral repair, the developed chondral microgels with high porosity and hydrophilicity could make cells spherical, favor the formation of cell aggregates, and show an excellent differentiation effect toward hyaline-like cartilage, thus contributing to the production of chondral microtissues. For subchondral bone repair, the fabricated subchondral microgels realize cell adhesion and proliferation and support the osteogenic differentiation of stem cells, thus favoring the formation of subchondral bone microtissues. The injectable chondral and subchondral bone microtissues could be stably assembled by Michael addition reaction between sulfhydryl groups of microtissues and double bonds of hydrophilic macromolecular cross-linker. At 12 weeks postimplantation, osteochondral microtissues could support the reconstruction of osteochondral-like tissues. The present study provides new insight into the microtissues for repair of osteochondral tissues.