Beneficial effects of aloperine on inflammation and oxidative stress by suppressing necroptosis in lipopolysaccharide-induced acute lung injury mouse model

坏死性下垂 脂多糖 炎症 氧化应激 化学 程序性细胞死亡 药理学 肿瘤坏死因子α 细胞凋亡 免疫学 分子生物学 医学 生物化学 生物
作者
Yanru Cui,Fei Qu,Wenjing Zhong,Hui‐Hui Yang,Jie Zeng,Junhao Huang,Jie Liu,Mingyue Zhang,Yong Zhou,Cha‐Xiang Guan
出处
期刊:Phytomedicine [Elsevier BV]
卷期号:100: 154074-154074 被引量:29
标识
DOI:10.1016/j.phymed.2022.154074
摘要

Alveolar epithelial cell death, inflammation, and oxidative stress are typical features of acute lung injury (ALI). Aloperine (Alo), an alkaloid isolated from Sophora alopecuroides, has been reported to display various biological effects, such as anti-inflammatory, immunoregulatory, and anti-oxidant properties. In this study, we investigated the effects and mechanisms of Alo in treating a lipopolysaccharide (LPS)-induced ALI in a murine model.The effects of Alo in LPS-induced ALI were investigated in C57BL/6 mice. The RIPK1 inhibitor (Nec-1) and the RIPK3 inhibitor (GSK'872) were used to evaluate the relationship of necroptosis, NF-κB activation, and PDC subunits in LPS-treated mouse alveolar epithelial cells (MLE-12). Then the effects of Alo on necroptosis, inflammation, and oxidative stress of LPS-stimulated MLE-12 cells were evaluated.Alo significantly attenuated histopathological lung injuries and reduced lung wet/dry ratio in LPS-induced ALI mice. Alo also remarkedly reduced total protein and neutrophils recruitment in bronchoalveolar lavage fluid of ALI mice. Meanwhile, Alo ameliorated the LPS-induced necroptosis in the lungs of ALI mice. The RIPK3 inhibitor GSK'872, but not the RIPK1 inhibitor Nec-1, reversed LPS-induced p65 phosphorylation and translocation to the nucleus in MLE-12 cells. GSK'872 also reversed the LPS-induced increase in ROS and binding of RIPK3 and PDC subunits in MLE-12 cells. Moreover, Alo down-regulated the levels of p-RIPK1, p-RIPK3, p-MLKL, p-p65, the translocation of p65 to the nucleus, and reduced the expression of IL-6 and IL-8 in LPS-stimulated MLE-12 cells. Alo also inhibited the binding of RIPK3 and PDC-E1α, PDC-E1β, PDC-E2, and PDC-E3 and the ROS production in LPS-treated MLE-12 cells.The present study validated the beneficial effects of Alo on LPS-induced ALI , suggesting Alo may be a new drug candidate against ALI.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
mary发布了新的文献求助10
1秒前
研友_VZG7GZ应助hahaha123213123采纳,获得10
1秒前
枕星发布了新的文献求助10
3秒前
3秒前
4秒前
4秒前
求求大家了完成签到,获得积分10
4秒前
阳光完成签到,获得积分10
4秒前
Crystal完成签到 ,获得积分10
6秒前
7秒前
7秒前
8秒前
8秒前
8秒前
乐乐应助赖道之采纳,获得10
9秒前
9秒前
Sun_Chen完成签到,获得积分10
9秒前
体贴凌柏发布了新的文献求助10
10秒前
成就的笑南完成签到 ,获得积分10
10秒前
11秒前
11秒前
wyw123完成签到,获得积分10
11秒前
求大佬帮助完成签到,获得积分10
11秒前
李健的小迷弟应助zyq采纳,获得10
12秒前
陈隆完成签到,获得积分10
12秒前
哎呀完成签到 ,获得积分10
12秒前
量子星尘发布了新的文献求助10
13秒前
mary完成签到,获得积分10
13秒前
13秒前
朱成豪发布了新的文献求助10
15秒前
deallyxyz应助科研通管家采纳,获得10
15秒前
科目三应助科研通管家采纳,获得10
15秒前
大个应助科研通管家采纳,获得10
15秒前
比比谁的速度快应助曾珍采纳,获得50
15秒前
15秒前
予修应助科研通管家采纳,获得10
15秒前
搜集达人应助科研通管家采纳,获得10
15秒前
wanci应助科研通管家采纳,获得30
15秒前
15秒前
15秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038368
求助须知:如何正确求助?哪些是违规求助? 3576068
关于积分的说明 11374313
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029