亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Beneficial effects of aloperine on inflammation and oxidative stress by suppressing necroptosis in lipopolysaccharide-induced acute lung injury mouse model

坏死性下垂 脂多糖 炎症 氧化应激 化学 程序性细胞死亡 药理学 肿瘤坏死因子α 细胞凋亡 免疫学 分子生物学 医学 生物化学 生物
作者
Yanru Cui,Fei Qu,Wenjing Zhong,Hui‐Hui Yang,Jie Zeng,Junhao Huang,Jie Liu,Mingyue Zhang,Yong Zhou,Cha‐Xiang Guan
出处
期刊:Phytomedicine [Elsevier BV]
卷期号:100: 154074-154074 被引量:29
标识
DOI:10.1016/j.phymed.2022.154074
摘要

Alveolar epithelial cell death, inflammation, and oxidative stress are typical features of acute lung injury (ALI). Aloperine (Alo), an alkaloid isolated from Sophora alopecuroides, has been reported to display various biological effects, such as anti-inflammatory, immunoregulatory, and anti-oxidant properties. In this study, we investigated the effects and mechanisms of Alo in treating a lipopolysaccharide (LPS)-induced ALI in a murine model.The effects of Alo in LPS-induced ALI were investigated in C57BL/6 mice. The RIPK1 inhibitor (Nec-1) and the RIPK3 inhibitor (GSK'872) were used to evaluate the relationship of necroptosis, NF-κB activation, and PDC subunits in LPS-treated mouse alveolar epithelial cells (MLE-12). Then the effects of Alo on necroptosis, inflammation, and oxidative stress of LPS-stimulated MLE-12 cells were evaluated.Alo significantly attenuated histopathological lung injuries and reduced lung wet/dry ratio in LPS-induced ALI mice. Alo also remarkedly reduced total protein and neutrophils recruitment in bronchoalveolar lavage fluid of ALI mice. Meanwhile, Alo ameliorated the LPS-induced necroptosis in the lungs of ALI mice. The RIPK3 inhibitor GSK'872, but not the RIPK1 inhibitor Nec-1, reversed LPS-induced p65 phosphorylation and translocation to the nucleus in MLE-12 cells. GSK'872 also reversed the LPS-induced increase in ROS and binding of RIPK3 and PDC subunits in MLE-12 cells. Moreover, Alo down-regulated the levels of p-RIPK1, p-RIPK3, p-MLKL, p-p65, the translocation of p65 to the nucleus, and reduced the expression of IL-6 and IL-8 in LPS-stimulated MLE-12 cells. Alo also inhibited the binding of RIPK3 and PDC-E1α, PDC-E1β, PDC-E2, and PDC-E3 and the ROS production in LPS-treated MLE-12 cells.The present study validated the beneficial effects of Alo on LPS-induced ALI , suggesting Alo may be a new drug candidate against ALI.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
量子星尘发布了新的文献求助10
7秒前
8秒前
黑大侠完成签到 ,获得积分10
9秒前
英姑应助lf采纳,获得10
9秒前
长街发布了新的文献求助20
9秒前
芋头粽子发布了新的文献求助30
11秒前
15秒前
欧皇发布了新的文献求助30
18秒前
lf发布了新的文献求助10
22秒前
scanker1981完成签到,获得积分10
23秒前
桐桐应助bbdd2334采纳,获得10
29秒前
30秒前
欧皇完成签到,获得积分20
32秒前
冷傲新柔发布了新的文献求助10
36秒前
37秒前
44秒前
HunterKK7发布了新的文献求助10
49秒前
轻松的惜芹应助yujie采纳,获得200
53秒前
沙雕续命完成签到,获得积分10
1分钟前
1分钟前
1分钟前
漂亮采波发布了新的文献求助10
1分钟前
开朗满天发布了新的文献求助10
1分钟前
1分钟前
华仔应助明亮猫咪采纳,获得10
1分钟前
搜集达人应助西域小飞侠采纳,获得10
1分钟前
Ava应助科研通管家采纳,获得10
1分钟前
赘婿应助科研通管家采纳,获得10
1分钟前
酷波er应助HunterKK7采纳,获得10
1分钟前
SciGPT应助开朗满天采纳,获得10
1分钟前
太清完成签到,获得积分10
1分钟前
lwm不想看文献完成签到 ,获得积分10
1分钟前
Djnsbj发布了新的文献求助10
1分钟前
闪闪蜜粉完成签到 ,获得积分10
1分钟前
NexusExplorer应助lf采纳,获得10
1分钟前
研友_VZG7GZ应助zzq采纳,获得10
1分钟前
小马甲应助执着的夜蓉采纳,获得10
1分钟前
1分钟前
oleskarabach完成签到,获得积分20
1分钟前
1分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976608
求助须知:如何正确求助?哪些是违规求助? 3520720
关于积分的说明 11204567
捐赠科研通 3257359
什么是DOI,文献DOI怎么找? 1798716
邀请新用户注册赠送积分活动 877897
科研通“疑难数据库(出版商)”最低求助积分说明 806613