白细胞介素10
小岛
白细胞介素4
下调和上调
促炎细胞因子
免疫系统
内分泌学
内科学
肠内分泌细胞
渗透(HVAC)
细胞因子
免疫学
医学
生物
内分泌系统
胰岛素
炎症
激素
生物化学
物理
基因
热力学
作者
Susanne Pfeiffer,Estefanía Quesada‐Masachs,Sara McArdle,Samuel Zilberman,Burçak Yesildag,Zbigniew Mikulski,Matthias von Herrath
标识
DOI:10.1016/j.clim.2022.109076
摘要
We defined the effect of the anti-inflammatory cytokines IL4 and IL10 on an in vitro model of human T1D. After preincubation with IL4 or IL10, human islet microtissues were co-cultured with PBMC and proinflammatory cytokines for a few hours or for multiple days to assess acute and chronic effects. This resulted in an immune attack with infiltration of T cells into the islet, a loss of beta cell endocrine function, and an upregulation of HLA-I on the beta cells. HLA-I upregulation was associated with infiltration of T cells and both HLA-I expression and infiltration were associated with impaired insulin secretion. Preincubation with IL4 or IL10 did not preserve beta cell function but decreased infiltration of T cells. Our data support the hypothesis that a loss of beta cell endocrine function mediates an increase in beta cell specific antigen presentation. IL4 and IL10 failed to preserve beta cell endocrine function.
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