Insights gained from Single-Cell analysis of immune cells on Cyclosporine A treatment in autoimmune uveitis

免疫系统 免疫学 自身免疫性疾病 生物 T细胞 钙调神经磷酸酶 免疫抑制 抗体 移植 医学 内科学
作者
Runping Duan,Lihui Xie,He Li,Rong Wang,Xiuxing Liu,Tianyu Tao,Shizhao Yang,Yuehan Gao,Xianchai Lin,Wenru Su
出处
期刊:Biochemical Pharmacology [Elsevier]
卷期号:202: 115116-115116 被引量:8
标识
DOI:10.1016/j.bcp.2022.115116
摘要

Cyclosporine A (CsA) is a widely known immunosuppressive agent that is clinically important in autoimmune diseases owing to its selective suppression of T lymphocytes. Although it has long been recognized to inhibit T cell responses by blocking calcineurin, the potential targets and specific downstream mechanisms remain elusive. Herein, we built a comprehensive single-cell transcriptomic landscape of immune cells in the blank, untreated experimental autoimmune uveitis (EAU), and CsA-treated EAU mice. CsA reversed EAU-associated changes in cell type composition, genomic expression, cell trajectory, and cell-cell communication. We found that CsA reverses the proportion change of disease-related immune cells; regulates several crucial pathogenic factors (eg. IL1r1, CD48, and Bhlhe40) in T helper 17 cells (Th17), the transcription factor Bhlhe40 was also rescued in T helper 1 cells (Th1); and may differentiate Tregs into a state of enhanced immunosuppression. In addition, we revealed the rescued impact of CsA on all immune cell types, especially on plasma B cells differentiation and immunoglobulin secretion. Furthermore, comparisons with glucocorticoids showed that CsA might have a more premium rescue effect involved in attenuating the pathogenicity of autoreactive T cells. Our work provides a comprehensive single-cell transcriptional atlas of immune cells under CsA therapy, providing advanced insights into the mechanisms underlying CsA and a reference for developing new therapeutic strategies for autoimmune diseases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刘晶发布了新的文献求助10
1秒前
1秒前
1秒前
1秒前
2秒前
霸气的猪发布了新的文献求助10
3秒前
4秒前
heya发布了新的文献求助10
5秒前
5秒前
LEO完成签到,获得积分10
5秒前
6秒前
6秒前
小小发布了新的文献求助10
6秒前
dao发布了新的文献求助10
7秒前
封尘逸动发布了新的文献求助10
7秒前
7秒前
llzuo发布了新的文献求助10
7秒前
8秒前
9秒前
10秒前
lg16完成签到,获得积分10
10秒前
11秒前
binshier完成签到,获得积分10
12秒前
12秒前
岁岁安完成签到,获得积分10
13秒前
13秒前
13秒前
14秒前
Luoyi完成签到,获得积分10
14秒前
义气代梅发布了新的文献求助10
16秒前
16秒前
大雷完成签到,获得积分10
16秒前
16秒前
17秒前
慕青应助GUGU采纳,获得10
17秒前
义气妙之发布了新的文献求助10
18秒前
18秒前
18秒前
18秒前
18秒前
高分求助中
Continuum Thermodynamics and Material Modelling 4000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Unseen Mendieta: The Unpublished Works of Ana Mendieta 1000
El viaje de una vida: Memorias de María Lecea 800
Luis Lacasa - Sobre esto y aquello 700
Novel synthetic routes for multiple bond formation between Si, Ge, and Sn and the d- and p-block elements 700
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3515227
求助须知:如何正确求助?哪些是违规求助? 3097638
关于积分的说明 9236245
捐赠科研通 2792536
什么是DOI,文献DOI怎么找? 1532575
邀请新用户注册赠送积分活动 712185
科研通“疑难数据库(出版商)”最低求助积分说明 707160