Mechanisms in photosynthesis are the key and fundamental cutting-edge question in life sciences. Their applications are closely related to the sustainability of agriculture, energy, and environment. This article briefly summarizes recent advances in several areas of photosynthesis research, including structure and regulation of photosynthetic protein machineries, photosynthetic water splitting and oxygen production and artificial photosynthesis, plant light energy utilization, carbon dioxide assimilation, and synthetic biology in photosynthesis, and proposes research perspectives. Meanwhile, this article discusses the challenges and proposes important research areas and directions in photosynthesis research until the year of 2035 in China.