内质网
细胞凋亡
未折叠蛋白反应
SH-SY5Y型
细胞生物学
切碎
p38丝裂原活化蛋白激酶
化学
生物
磷酸化
分子生物学
细胞培养
生物化学
遗传学
神经母细胞瘤
MAPK/ERK通路
作者
Mei Zhang,Ying Wang,Ricky M S Wong,Ken Kin Lam Yung,Ruijin Li
标识
DOI:10.1016/j.neuro.2021.11.012
摘要
Exposure to ambient fine particulate matter (PM2.5) may contribute to brain injury, however, the molecular mechanisms have not yet been fully described. In this study, the human SH-SY5Y cells were treated with PM2.5 with different concentrations (0, 25, 100, and 250 μg/mL) for 24 h to investigate the cell apoptosis mediated by endoplasmic reticulum (ER) stress. The ratio of apoptosis, Ca2+ level, biomarkers of ER stress and apoptosis were determined. The results revealed that PM2.5 triggered the increase of apoptosis ratio and cellular Ca2+ levels. Compared with control, the expression of GRP78 and phosphorylation of IER1α and p38 were enhanced significantly in the cells under the conditions of PM2.5 exposure for activating ER stress signals. Besides, the key genes (CHOP/DR5/Caspase8/Caspase12) in ER stress-induced apoptosis signals were up-regulated after the PM2.5 treatment compared to the control. The results suggested PM2.5 induced apoptosis in SH-SY5Y cells by the stimulation of ER stress, which may be the potential mechanism of neurological diseases incurred by PM2.5.
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