Facile synthesis of reduced graphene oxide using Acalypha indica and Raphanus sativus extracts and their in vitro cytotoxicity activity against human breast (MCF-7) and lung (A549) cancer cell lines

In the present study, an eco-friendly approach is adapted for the synthesis of reduced graphene oxide (rGO's) by a simple hydrothermal reaction using two plant extracts namely Acalypha indica and Raphanus sativus. After the hydrothermal reaction, GO turns into a black color from brown color, which indicates the successful reduction of graphene oxide. Further, various characterization techniques such as UV-Vis spectroscopy, Raman spectroscopy, Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction is used to confirm the physicochemical properties of synthesized rGO's. Raman analysis confirms the reduction of GO by noticing an increase in the ID/IG ratio significantly. Field emission scanning electron microscopy and transmission electron microscopy clearly show the morphology and crystalline nature of rGO's. FT-IR spectrum confirms that the bioactive molecules of the plant extract (i.e. polyphenols, flavonoids, terpenoids, etc.) playing a key role in the elimination of oxygen groups from the GO surface. Further, the synthesized rGO's are tested for their potential against human lung and breast cancer cell lines. A significant cancer cell inhibition activity is obtained even in the less concentration of rGO's with IC50 values for lung cancer cell lines are 38.46 µg/mL and 26.69 µg/mL for AIrGO and RSrGO, respectively. Similarly, IC50 values for breast cancer cell lines are 35.97 µg/mL and 33.22 µg/mL for AIrGO and RSrGO, respectively.