It is of great significance to acquire seismic signal accurately in seismic observation and research. A seismic data acquisition system based on high-precision data acquisition card NI USB-4432 and LabVIEW platform is designed and implemented. Firstly, the seismic signal is modulated by the front-end conditioning circuit composed of AD620 and MAX292, then, it is obtained and analyzed by data acquisition system based on USB-4432 and LabVIEW. To improve the signal quality and promote the signal-to-noise ratio (SNR), an improved wavelet threshold de-noising algorithm is proposed, which can de-noise adaptively according to the intensity of the collected signal. Practical test results show that, the designed system characterizes high stability, can effectively improve the SNR, and possesses a higher applied value.