Autotrophic nitrogen removal in an integrated fixed-biofilm activated sludge (IFAS) reactor: Anammox bacteria enriched in the flocs have been overlooked

Abstract In this study, an autotrophic nitrogen removal process was established using an integrated fixed-biofilm activated sludge (IFAS) reactor treated with high ammonium wastewater. A nitrogen removal rate (NRR) of 2.78 kg N/(m3·d) was obtained during the 206-day operation. Moreover, during the stable period, the large flocs (D > 0.2 mm) had a significantly higher abundance of anammox bacteria than the small flocs (D