Tetrabromobisphenol A (TBBPA) inhibits denitrification via regulating carbon metabolism to decrease electron donation and bacterial population

The potential risks of brominated flame retardants (BFRs) like tetrabromobisphenol A (TBBPA) have attracted much attention. However, the influence of TBBPA on functional microbes remains poorly understood, especially with regards to denitrification, which is closely related with the carbon and nitrogen cycles, eutrophication and greenhouse gas emission. Herein, we found that 1.0 mg/L of TBBPA significantly decreased the total nitrogen removal efficiency by 81.7%, but increased the accumulation of NO2−–N (by 81.5%) and N2O (by 172-fold). This was found to be underlie by the significant decrease in both the denitrifying capability of denitrifiers and total bacterial population. Further investigation revealed that TBBPA inhibited the pathways of glucolysis and pentose phosphate, and promoted glyoxylate bypass via regulating genes expressions of key enzymes (such as glucose-6-phosphate isomerase, pyruvate dehydrogenase, isocitrate lyase, etc.), then decreased the generation of NADH serving as electron donor for denitrification, and inhibited the denitrifying capability of denitrifiers. Moreover, insufficient NADH stimulated the accumulation of denitrifying intermediates (NO2−–N and N2O), which induced the increase of reactive nitrogen species (RNS), whose accumulation decreased proliferation and increased apoptosis of denitrifying bacteria. Finally, the decrease in the denitrifying capability of denitrifier and bacterial population resulted in negative denitrifying performance.