ABCA1
安普克
细胞凋亡
ABCG1公司
泡沫电池
细胞生物学
下调和上调
CYP27A1
Wnt信号通路
化学
胆固醇
生物
信号转导
蛋白激酶A
生物化学
激酶
脂蛋白
运输机
基因
作者
Shengyun Lei,Jiying Chen,Chengxiang Song,Jun Li,Anju Zuo,Dan Xu,Ting-Ting Li,Yuan Guo
标识
DOI:10.1016/j.mce.2020.111138
摘要
The apoptosis of foam cells leads to instability of atherosclerotic plaques. This study was designed to explore the protective role of CTRP9 in foam cell apoptosis. In our experiment, CTRP9 alleviated foam cell apoptosis. Meanwhile, CTRP9 upregulated the expression of proteins important for cholesterol efflux, such as LXRα, CYP27A1, ABCG1 and ABCA1, and improved cholesterol efflux in foam cells. Moreover, CTRP9 inhibited Wnt3a and β-catenin expression and β-catenin nuclear translocation in foam cells. In addition, adenovirus overexpression of Wnt3a abolished the effect of CTRP9 on macrophage apoptosis. Mechanistically, the AMPK inhibitor abolished the effect of CTRP9 on foam cell apoptosis, and downregulation of AdipoR1 by siRNA abrogated the activation of AMPK and the effect of CTRP9 on foam cell apoptosis. We concluded that CTRP9 achieved these protective effects on foam cells through the AdipoR1/AMPK pathway. • CTRP9 alleviates foam cells apoptosis by enhancing cholesterol efflux. • CTRP9 upregulated ABCA1 expression through Wnt/β-catenin pathway. • The effects of CTRP9 on Wnt/β-catenin pathway mediated by AdipoR1/AMPK.
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