FGF21型
自噬
内科学
非诺贝特
内分泌学
炎症
糖尿病性心肌病
糖尿病
西妥因1
2型糖尿病
纤维化
链脲佐菌素
医学
心脏纤维化
锡尔图因
氧化应激
兴奋剂
下调和上调
化学
受体
心力衰竭
成纤维细胞生长因子
细胞凋亡
心肌病
乙酰化
基因
生物化学
作者
Jingjing Zhang,Yanli Cheng,Junlian Gu,Shudong Wang,Shanshan Zhou,Yuehui Wang,Yi Tan,Wenke Feng,Yaowen Fu,Nicholas Mellen,Rui Cheng,Jian‐xing Ma,Chi Zhang,Zhanquan Li,Lu Cai
出处
期刊:Clinical Science
[Portland Press]
日期:2016-03-08
卷期号:130 (8): 625-641
被引量:140
摘要
Fenofibrate (FF), as a peroxisome-proliferator-activated receptor α (PPARα) agonist, has been used clinically for decades to lower lipid levels. In the present study, we examined whether FF can be repurposed to prevent the pathogenesi of the heart in Type 1 diabetes and to describe the underlying mechanism of its action. Streptozotocin (STZ)-induced diabetic mice and their age-matched control mice were treated with vehicle or FF by gavage every other day for 3 or 6 months. FF prevented diabetes-induced cardiac dysfunction (e.g. decreased ejection fraction and hypertrophy), inflammation and remodelling. FF also increased cardiac expression of fibroblast growth factor 21 (FGF21) and sirtuin 1 (Sirt1) in non-diabetic and diabetic conditions. Deletion of FGF21 gene (FGF21-KO) worsened diabetes-induced pathogenic effects in the heart. FF treatment prevented heart deterioration in the wild-type diabetic mice, but could not do so in the FGF21-KO diabetic mice although the systemic lipid profile was lowered in both wild-type and FGF21-KO diabetic mice. Mechanistically, FF treatment prevented diabetes-impaired autophagy, reflected by increased microtubule-associated protein 1A/1B-light chain 3, in the wild-type diabetic mice but not in the FGF21-KO diabetic mice. Studies with H9C2 cells in vitro demonstrated that exposure to high glucose (HG) significantly increased inflammatory response, oxidative stress and pro-fibrotic response and also significantly inhibited autophagy. These effects of HG were prevented by FF treatment. Inhibition of either autophagy by 3-methyladenine (3MA) or Sirt1 by sirtinol (SI) abolished FF's prevention of HG-induced effects. These results suggested that FF could prevent Type 1 diabetes-induced pathological and functional abnormalities of the heart by increasing FGF21 that may up-regulate Sirt1-mediated autophagy.
科研通智能强力驱动
Strongly Powered by AbleSci AI