Changes in the expression of c-fos & heat shock protein genes & blood flow velocity in the brain of rats undergoing myocardial ischaemia/reperfusion.

脑血流 热休克蛋白 海马体 热休克蛋白70 缺血 内科学 医学 休克(循环) 再灌注损伤 麻醉 血流 心脏病学 病理 内分泌学 生物 基因 生物化学
作者
Shuguang Wang,Xiaoling Xu,Li Gu
出处
期刊:PubMed 卷期号:123 (2): 131-8 被引量:3
链接
标识
摘要

Myocardial hypofunction could lead to the derangement of brain functions. The expression of c-fos and heat shock protein (hsp) genes was recognized as markers of neural cell injury. We under took this study to investigate the influence of myocardial ischaemia and reperfusion (I/R) on these molecular events in the rat brain tissue and changes in mean arterial blood pressure (MAP) and the cerebral blood flow velocity (CBFV), to understand the basis of cerebral pathology following cardiac ischaemia and reperfusion.Healthy rats (n=42) were randomly allocated into seven groups: sham-operated (A); myocardial ischaemia for 15 min, followed by 0.5, 1, 2, 4 and 6 h of reperfusion respectively (B2-B6) and hypovolemia at medium level (C). I/R rat models were established by ligating the anterior branch of the left coronary artery. Expressions of the c-fos, hsp70, hsp27 and hsp90 genes in the cerebrum, cerebellum, medullaoblongata and hippocampus, were studied with immunohistochemistry and in situ hybridization. The MAP and CBFV of the rats were also measured.The expressions of c-fos and HSP70 in brain tissue increased after myocardial ischaemia/ reperfusion, with the strongest signal appearing in the hippocampus and cerebral cortex, while labeling for HSP27 and HSP90 alpha were not detectable in any of the experimental groups. The expressions of c-fos mRNA and hsp70 mRNA shared the similar characteristics with their encoding proteins. MAP, reflection of cardiac function and cerebral blood flow decreased following cardiac ischaemia and reperfusion.Our findings suggested that the brain damages occurred from the early phase of myocardial I/R. The exact mechanism of cerebral tissue injuries induced by myocardial I/R is not known. Further studies need to be done to throw light on these aspects.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
天天完成签到 ,获得积分10
刚刚
土土完成签到,获得积分10
1秒前
joyi发布了新的文献求助10
1秒前
fan完成签到 ,获得积分10
1秒前
华仔应助lili采纳,获得30
2秒前
3秒前
852应助科研小菜喵采纳,获得10
3秒前
黙宇循光发布了新的文献求助10
4秒前
流年xy完成签到,获得积分10
4秒前
5秒前
6秒前
6秒前
自然的qm完成签到,获得积分10
6秒前
weiyu_u发布了新的文献求助30
8秒前
zzz发布了新的文献求助10
8秒前
猪猪朱发布了新的文献求助30
8秒前
10秒前
zhong完成签到,获得积分10
10秒前
大气的念薇完成签到 ,获得积分10
10秒前
伶俐曼寒发布了新的文献求助10
11秒前
称心玉米发布了新的文献求助10
13秒前
14秒前
15秒前
15秒前
知鸢完成签到 ,获得积分10
15秒前
黄风小圣完成签到 ,获得积分10
16秒前
圈儿完成签到,获得积分10
17秒前
17秒前
8R60d8应助ly普鲁卡因采纳,获得10
17秒前
19秒前
19秒前
19秒前
一与余完成签到,获得积分10
19秒前
jeep先生发布了新的文献求助10
20秒前
独行侠发布了新的文献求助10
21秒前
过分动真完成签到 ,获得积分10
22秒前
nove999完成签到 ,获得积分10
23秒前
hyhyhyhy发布了新的文献求助10
23秒前
art888完成签到,获得积分10
24秒前
25秒前
高分求助中
The Oxford Handbook of Social Cognition (Second Edition, 2024) 1050
Kinetics of the Esterification Between 2-[(4-hydroxybutoxy)carbonyl] Benzoic Acid with 1,4-Butanediol: Tetrabutyl Orthotitanate as Catalyst 1000
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
Handbook of Qualitative Cross-Cultural Research Methods 600
Chen Hansheng: China’s Last Romantic Revolutionary 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3140111
求助须知:如何正确求助?哪些是违规求助? 2790982
关于积分的说明 7797203
捐赠科研通 2447324
什么是DOI,文献DOI怎么找? 1301841
科研通“疑难数据库(出版商)”最低求助积分说明 626345
版权声明 601194