NEW PLANTLET PROLIFERATION AND BULBING PROMOTION IN IN VITRO CULTURE OF ORNITHOGALUM HYBRID

The in vitro culture on Ornithogalum hybrid cultivar was investigated for mass propagation. The highest development of shoot number was observed when bulb scale explants cultured on MS medium under continuous fluorescent light. In general, culturing under light condition promoted shoot and root development more than darkness. The number of shoot was significantly increased when perianth as a source of explant was cultured at 0.5 mg·L -1 NAA and 1.5 mg·L -1 BA combination. The proximal position of leaf explants showed superior responses, increasing of shoot number to 11.2. A temperature of 19°C was found to be most effective in the increasing of shoot number at 5.4. The development of shoot and root was inhibited at lower culture temperature. The number of shoot and root were promoted on the medium of mixed with 60g·L -1 sucrose and 0.8 mg·L -1 ancymidol. However, development of plantlet was not affected by high concentration of 60 g·L -1 sucrose. The diameter, fresh and dry weight of bulb to tend promote at 35/15°(D/N). In conclusion, when proximal position of leaf explants were cultured on MS basic medium, supplemented with 30 g·L -1 sucrose, 0.8 mg·L -1 ancymidol, and 0.5 mg·L -1 NAA and 1.5 mg·L -1 BA , and incubate at 19°C under continuous white light condition, the highest mean number of shoots was showed. And those results with 35/15°C treatment at the stage of bulbing in vitro culture were considered that rapid bulb production is possible.