去酰胺
化学
丁二酰亚胺
酰亚胺
肽
聚乙二醇
溶剂
水溶液
结晶
有机溶剂
环肽
有机化学
高分子化学
生物化学
化学工程
酶
工程类
作者
Sante Capasso,L. Mazzarella,Adriana Zagari
出处
期刊:PubMed
日期:1991-07-01
卷期号:4 (4): 234-8
被引量:47
摘要
The deamidation reaction of Asn side chain was studied in the presence of the chemicals usually used at high concentration in the purification and crystallization of peptides and proteins. All the experiments were performed on the model peptide Boc-L-Asn-Gly-Gly-NH2. The pathway of the reaction is not affected by the medium used; in all cases it proceeds through a succinimide intermediate giving a mixture of isoaspartyl and aspartyl peptide. However, the rate of the reaction significantly depends on the solvent: the addition of organic solvents to an aqueous solution of the peptide has the general effect of decreasing the reaction rate, which, on the other hand, is strongly enhanced by a high concentration of organic and inorganic buffers. Only a minor influence is exerted by aprotic salts and polyethylene glycol.
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