Study on the Nondestructive Method to Sample and Extract Total RNA from Hair Follicle in Muscovy Duck

Objective:To find an efficient method for the extraction of total RNA from hair follicle tissues of muscovy duck.Methods:The effects of nondestructive samples and destructive samples on total RNA extraction were investigated.The total RNA extracted by improving TRIzol method in grinding and homogenate in details was analysed by agarose electrophoresis.Results:The two kinds of hair follicle sampling methods both can extract high-quality total RNA.There were three bands of 28S,18S and 5S obviously,and no DNA stain in total RNA. Conclusion:The nondestructive samples were suitable methods used to extract total RNA from hair follicle of muscovy duck.