First Report of Convolvulus arvensis and Polycarpon tetraphyllum as Natural Hosts of Tomato Brown Rugose Fruit Virus

HomePlant DiseaseAhead of PrintFirst Report of Convolvulus arvensis and Polycarpon tetraphyllum as Natural Hosts of Tomato Brown Rugose Fruit Virus PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Convolvulus arvensis and Polycarpon tetraphyllum as Natural Hosts of Tomato Brown Rugose Fruit VirusM. Cultrona, N. Bonini, D. Pacifico, and M. TessitoriM. CultronaDepartment of Agriculture, Food and Environment (Di3A), University of Catania, 95123 Catania, Italy, N. BoniniDepartment of Agriculture, Food and Environment (Di3A), University of Catania, 95123 Catania, Italy, D. PacificoInstitute of Biosciences and BioResources, National Council of Research (IBBR-CNR), Division of Palermo, 90129 Palermo, Italy, and M. Tessitori†Corresponding author: M. Tessitori; E-mail Address: [email protected]https://orcid.org/0000-0003-4285-8569Department of Agriculture, Food and Environment (Di3A), University of Catania, 95123 Catania, ItalyAffiliationsAuthors and Affiliations M. Cultrona1 N. Bonini1 D. Pacifico2 M. Tessitori1 † 1Department of Agriculture, Food and Environment (Di3A), University of Catania, 95123 Catania, Italy 2Institute of Biosciences and BioResources, National Council of Research (IBBR-CNR), Division of Palermo, 90129 Palermo, Italy Published Online:4 Mar 2024https://doi.org/10.1094/PDIS-11-23-2413-PDNAboutSectionsPDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Tomato brown rugose fruit virus (ToBRFV) is a tobamovirus recently identified on tomatoes in Jordan (Salem et al. 2016). New infections were rapidly reported all over the world, becoming a serious threat to tomato production. About 40 species belonging to four families (Amaranthaceae, Apocynaceae, Asteraceae, and Solanaceae) have been reported as experimental hosts (Salem et al. 2023). Tomato and pepper have been reported as natural hosts of ToBRFV, but recently Salem and coworkers (2022) detected the presence of the virus in 12 wild species. To identify potential natural hosts of the virus, 10 plants of bindweed (Convolvulus arvensis L.) and 7 of fourleaf allseed (Polycarpon tetraphyllum L.) were collected in the summer of 2023 in a tomato greenhouse located in Pachino, Siracuse Province (Sicily, Italy), with a high infection rate of ToBRFV. These two species were chosen because they were predominant among the spontaneous weeds inside the greenhouse. No symptoms ascribable to ToBRFV were observed on bindweed and fourleaf allseed during the surveys. All leaf samples were analyzed for ToBRFV infections by DAS-ELISA with a commercial antiserum (LOEWE Biochemica, Germany), including tomato positive and negative controls. Eight C. arvensis and seven P. tetraphyllum samples out of the total tested positive to ToBRFV. To confirm virus presence, total RNA was extracted from all samples using the RNeasy Plant Mini Kit (QIAGEN) and used as a template for RT-PCR with ToBRFV-specific primers (Alkowni et al. 2019). RT-PCR products of the expected size (560 bp) confirmed DAS-ELISA results. Amplicons from two isolates of each plant species (Conv-01, Conv-02, Poly-01, and Poly-02) were gel-purified and sequenced in both directions. Sequences were edited and deposited in GenBank (accession nos. Conv-01, OP150933; Conv-02, OP193999; Poly-01, OP150934; Poly-02, OP194000). According to sequence analysis, the four isolates shared 100% identity among them and 98.82% identity with the ToBRFV reference sequence (GenBank accession no. KT383474). To our knowledge, this is the first report of ToBRFV natural infections in C. arvensis and P. tetraphyllum. Since these weeds are common in our tomato production areas, they could act as ToBRFV reservoirs.The author(s) declare no conflict of interest.References:Alkowni, R., et al. 2019. J. Plant Pathol. 101:719. https://doi.org/10.1007/s42161-019-00240-7 Crossref, ISI, Google ScholarSalem, N., et al. 2016. Arch. Virol. 161:503. https://doi.org/10.1007/s00705-015-2677-7 Crossref, ISI, Google ScholarSalem, N. M., et al. 2022. Plants 11:2287. https://doi.org/10.3390/plants11172287 Crossref, Google ScholarSalem, N. M., et al. 2023. Annu. Rev. Phytopathol. 61:137-164. https://doi.org/10.1146/annurev-phyto-021622-120703 Crossref, ISI, Google ScholarFunding: This research was funded by the University of Catania "Investigation of Phytopathological Problems of the Main Sicilian Productive Contexts and Eco-Sustainable Defense Strategies" (PIA.CE.RI. 2020–2022) and by the INTERREG V-A Italia-Malta 2014-2020 Programme "FAST Project-Fight Alien Species Transborder."The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Just PublishedSubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Published: 4 Mar 2024First Look: 8 Jan 2024Accepted: 26 Dec 2023 Information© 2024 The American Phytopathological SocietyFundingUniversity of CataniaGrant/Award Number: PIA.CE.RI. 2020–2022INTERREG V-A Italia-Malta 2014-2020 ProgrammeKeywordsItalynatural hostsToBRFVThe author(s) declare no conflict of interest.PDF download