二次谐波产生
显微镜
光学
极化(电化学)
材料科学
分辨率(逻辑)
光学显微镜
衍射
胶原纤维
偏振光显微镜
偏振显微镜
扫描电子显微镜
化学
生物物理学
物理
激光器
生物
物理化学
人工智能
计算机科学
作者
Yong Zhang,Chenshuang Zhang,Renlong Zhang,Rong Xu,Bin Yu,Danying Lin,Junle Qu
出处
期刊:Optics Letters
[Optica Publishing Group]
日期:2024-02-21
卷期号:49 (6): 1540-1540
被引量:1
摘要
Polarization-resolved second-harmonic generation (PSHG) microscopy is widely used in investigating the structural and morphological alterations of collagen. However, the resolution of second-harmonic generation (SHG) imaging remains constrained by optical diffraction, resulting in the polarization extraction of collagen characteristics from the average properties of collagen fibers. In this study, multifocal structured illumination microscopy (MSIM) was combined with PSHG to achieve polarization-resolved super-resolution imaging of second-harmonic generation signals. For the first time to our knowledge, periodic structures with an average pitch of 277 nm were observed in mouse tail tendons using optical microscopy, and the orientation angle of fibrils within each period was found to exhibit an alternating arrangement along the axis in a regular pattern.
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