生物传感器
计算机科学
快速成型
纳米技术
嵌入式系统
计算生物学
生物
工程类
材料科学
机械工程
作者
Wonhee Kim,S. Lee,Bong Hyun Sung,Jeong‐Geol Na,Jeong Wook Lee
标识
DOI:10.1016/j.coisb.2023.100501
摘要
Integration of cell-free systems with genetic biosensors is emerging as an advantageous platform for small molecule detection. This biosensor-coupled cell-free system simplifies an assay-and-detection procedure by combining the advantages of rapid and efficient protein expression through a cell-free system and the in situ detection capabilities provided by genetic biosensors. Moreover, this system is easy to assay multiple conditions at once, as the open environment of the cell-free systems enhances overall ease of handling. In this review, we focus on the acceleration of enzyme and pathway prototyping using cell-free biosensors, as well as strategies to improve the sensitivity and specificity of biosensors. High-throughput screening tools that can expand the prototyping process by generating massive data sets for rapid evaluation were also described.
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