光学
斑点图案
显微镜
计算机科学
计算机视觉
空间频率
人工智能
图像质量
傅里叶变换
相位恢复
迭代重建
帧速率
可视化
物理
图像(数学)
量子力学
作者
Shijie Tu,Xiaoyan Li,Yueying Wang,Wenwen Gong,Xin Liu,Q. Liu,Yubing Han,Cuifang Kuang,Xü Liu,Xiang Hao
出处
期刊:Optics Letters
[The Optical Society]
日期:2023-04-03
卷期号:48 (10): 2535-2535
被引量:3
摘要
Structured illumination microscopy (SIM) allows non-invasive visualization of nanoscale subcellular structures. However, image acquisition and reconstruction become the bottleneck to further improve the imaging speed. Here, we propose a method to accelerate SIM imaging by combining the spatial re-modulation principle with Fourier domain filtering and using measured illumination patterns. This approach enables high-speed, high-quality imaging of dense subcellular structures using a conventional nine-frame SIM modality without phase estimation of the patterns. In addition, seven-frame SIM reconstruction and additional hardware acceleration further improve the imaging speed using our method. Furthermore, our method is also applicable to other spatially uncorrelated illumination patterns, such as distorted sinusoidal, multifocal, and speckle patterns.
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