Anatomical and Technical Preparations of the Human Brain for White Matter Fibers Dissection with Electromagnetic Neuronavigation Assistance Technical Nuances for Application

Klinger's fiber dissection technique is widely used for studying the anatomy of white matter. Herein, we present a technical description of Klinger's proposed fiber dissection algorithm with neuronavigation assistance which allows for a more accurate determination of the projection of association fibers.An anatomical study was conducted on 8 hemispheres of the human brain, prepared according to the Klingler fiber dissection technique. In all the cases, a frameless electromagnetic navigation system was used. For each anatomical specimen, an individualized support device was three-dimensional -printed and placed it into the magnetic resonance imaging (MRI) gantry. MRI study of each anatomical specimen was performed using a specific protocol that enabled a subsequent three-dimensional visualization of the anatomical structures as follows: FSPGR (Fast SPoiled Gradient Recalled echo) BRAVO (BRAin VOlume Imaging), T2 CUBE, FLAIR (FLuid Attenuated Inversion Recovery) CUBE, CUBE DIR (double inversion recovery) WHITE MATTER, and CUBE DIR GRAY MATTER.The average time required to register an anatomical specimen in the navigation system was 7 minutes 28 seconds. In all of the 8 cases, the anatomical structures were correctly identified using neuronavigation. Moreover, the choice of MRI mode depends on the purpose of the study and the region of interest in the brain.Electromagnetic navigation is an accurate and useful technique. It allows the researcher the ability to virtually project the association fibers and their cortico-cortical terminations to the surface of the brain, even at the final stages of dissection when the superficial structures are removed. To obtain accurate targeting, it is important to use the appropriate neuronavigation protocol.