Dual-emissive carbonized polymer dots for the ratiometric fluorescence imaging of singlet oxygen in living cells

单线态氧 光化学 化学 荧光 光敏剂 活性氧 氧气 有机化学 生物化学 量子力学 物理
作者
Cheng‐Ruei Yang,Yu‐Syuan Lin,Ren-Siang Wu,Chin‐Jung Lin,Han‐Wei Chu,Chih‐Ching Huang,Anisha Anand,Binesh Unnikrishnan,Huan‐Tsung Chang
出处
期刊:Journal of Colloid and Interface Science [Elsevier]
卷期号:634: 575-585 被引量:11
标识
DOI:10.1016/j.jcis.2022.12.076
摘要

Singlet oxygen (1O2) is a type of reactive oxygen species (ROS), playing a vital role in the physiological and pathophysiological processes. Specific probes for monitoring intracellular 1O2 still remain challenging. In this study, we develop a ratiometric fluorescent probe for the real-time intracellular detection of 1O2 using o-phenylenediamine-derived carbonized polymer dots (o-PD CPDs). The o-PD CPDs possessing dual-excitation-emission properties (blue and yellow fluorescence) were successfully synthesized in a two-phase system (water/acetonitrile) using an ionic liquid tetrabutylammonium hexafluorophosphate as a supporting electrolyte through the electrolysis of o-PD. The o-PD CPDs can act as a photosensitizer to produce 1O2 upon white LED irradiation, in turn, the generated 1O2 selectively quenches the yellow emission of the o-PD CPDs. This quenching behavior is ascribed to the specific cycloaddition reaction between 1O2 and alkene groups in the polymer scaffolds on o-PD CPDs. The interior carbon core can be a reliable internal standard since its blue fluorescence intensity remains unchanged in the presence of 1O2. The ratiometric response of o-PD CPDs is selective toward 1O2 against other ROS species. The developed o-PD CPDs have been successfully applied to monitor the 1O2 level in the intracellular environment. Furthermore, in the inflammatory neutrophil cell model, o-PD CPDs can also detect the 1O2 and other ROS species such as hypochlorous acid after phorbol 12-myristate 13-acetate (PMA)-induced inflammation. Through the dual-channel fluorescence imaging, the ratiometric response of o-PD CPDs shows great potential for detecting endogenous and stimulating 1O2 in vivo.
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