[Effects of Different Treatment Methods on the Contents of Related Growth Factors Released by Platelet Rich Plasma].

To evaluate the effect of sonication, repeated freeze-thaw cycles, calcium salt solution and their combination on the content of related growth factors (GFs) released by platelet rich plasma (PRP).Twenty PRPs from healthy blood donors were divided into 9 groups, including sonication group, freeze-thaw group, calcium gluconate group, calcium chloride group, sonication + calcium gluconate group, sonication + calcium chloride group, freeze-thaw + calcium gluconate group, freeze-thaw + calcium chloride group, and sonication + freeze-thaw group. After PRP activated by above 9 methods, the content of transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor (VEGF), and platelet-derived growth factor-BB (PDGF-BB) were detected by ELISA.The platelet concentration of the samples was (966.7±202.6)×109/L. The content of TGF-β1 in sonication + freeze-thaw group was the highest, while the lowest was in freeze-thaw group. The content of VEGF in freeze-thaw + calcium chloride group was the highest, while the lowest was in calcium gluconate group. The content of PDGF-BB in sonication + freeze-thaw group was the highest, while the lowest was in calcium gluconate group. There was no significant differences in the three GFs between calcium gluconate group and calcium chloride group.Among the 9 activated methods of PRP, there is no difference between two calcium salt solutions. And the combination of repeated freeze-thaw cycles and sonication may be the best treatment method to promote PRP to release GFs, while calcium gluconate is the weakest way.不同处理方式对富血小板血浆相关生长因子的影响.探究超声、冻融、钙离子及其组合等处理方法对富血小板血浆（PRP）释放生长因子含量的影响.20份PRP来自健康献血者，根据不同处理方式将每份分为9组，即超声组、冻融组、葡萄糖酸钙组、氯化钙组、超声+葡萄糖酸钙组、超声+氯化钙组、冻融+葡萄糖酸钙组、冻融+氯化钙组、超声+冻融组，采用ELISA检测每组处理后的PRP中转化生长因子β1（TGF-β1）、血管内皮生长因子（VEGF）、血小板衍生生长因子BB（PDGF-BB）的含量.样本的血小板浓度为(966.7±202.6)×109/L。TGF-β1含量最低的是冻融组，最高的是超声+冻融组；VEGF含量最低的是葡萄糖酸钙组，最高的是冻融+氯化钙组；PDGF-BB含量最低的是葡萄糖酸钙组，最高的是超声+冻融组。3种生长因子的含量在葡萄糖酸钙组与氯化钙组间比较均没有统计学差异（P>0.05）.9种PRP处理方式中，两种钙离子激活剂无差异；反复冻融及超声处理可能是促进PRP释放生长因子的最佳方式，葡萄糖酸钙则是作用最弱的方式.