芳香烃受体
苯并(a)芘
小RNA
外周血单个核细胞
基因表达
生物
致癌物
细胞凋亡
芘
核糖核酸
基因表达调控
细胞生物学
基因
分子生物学
化学
遗传学
体外
转录因子
天体生物学
作者
Rima Souki,Jérémy Amosse,Valentine Genêt,Morgane Le Gall,Benjamin SaintPierre,Franck Letourneur,Anne Maı̂tre,Christine Demeilliers,Eric Le Ferrec,Dominique Lagadic‐Gossmann,Normand Podechard,Lydie Sparfel
标识
DOI:10.1016/j.envpol.2023.121653
摘要
Polycyclic aromatic hydrocarbons (PAHs) are widely distributed environmental contaminants, triggering deleterious effects such as carcinogenicity and immunosuppression, and peripheral blood mononuclear cells (PBMCs) are among the main cell types targeted by these pollutants. In the present study, we sought to identify the expression profiles and function of miRNAs, gene regulators involved in major cellular processes recently linked to environmental pollutants, in PBMC-exposed to the prototypical PAH, benzo[a]pyrene (B[a]P). Using small RNA deep sequencing, we identified several B[a]P-responsive miRNAs. Bioinformatics analyses showed that their predicted targets could modulate biological processes relevant to cell death and survival. Further studies of the most highly induced miRNA, miR-132, showed that its up-regulation by B[a]P was time- and dose-dependent and required aryl hydrocarbon receptor (AhR) activation. By evaluating the role of miR-132 in B[a]P-induced cell death, we propose a mechanism linking B[a]P-induced miR-132 expression and cytochromes P-450 (CYPs) 1A1 and 1B1 mRNA levels, which could contribute to the apoptotic response of PBMCs. Altogether, this study increases our understanding of the roles of miRNAs induced by B[a]P and provides the basis for further investigations into the mechanisms of gene expression regulation by PAHs.
科研通智能强力驱动
Strongly Powered by AbleSci AI