203.3: Reducing neutral protease dose during islet isolation does not impair islet recovery from pancreases retrieved for autologous use

小岛 胶原酶 医学 胰腺 移植 内科学 蛋白酶 泌尿科 糖尿病 男科 内分泌学 化学 生物化学
作者
Rebecca M Spiers,Heide Brandhorst,Daniel Brandhorst,Idira Obasse,Jennifer M. Fox,Alistair Lumb,Michael Silva,Paul Johnson
出处
期刊:Transplantation [Wolters Kluwer]
卷期号:107 (10S2): 43-43
标识
DOI:10.1097/01.tp.0000993972.83483.b5
摘要

Introduction: Total Pancreatectomy with Islet Auto-Transplantation (TPIAT) is an effective treatment for selected patients with severe chronic pancreatitis, resulting in pain reduction, improved quality of life, and good graft function following islet re-infusion. Islets are isolated using a blend of collagenase and neutral protease (NP) enzymes. Most islet isolation labs use high concentrations of NP in auto islet isolation (~200 Units), due to the fibrotic nature of the retrieved pancreases. However, increasing concentrations of NP can result in islet fragmentation and reduce islet viability. Here, we investigate the impact of using a lower protease dose on islet isolation outcome, from pancreases retrieved for autologous use. Methods: Following total pancreatectomy, islets were isolated using the semi-automated method. Enzymes mixtures consisted of Vitacyte collagenase (~2,000 Units) and either 100 Units (U) or 200 U of Serva/Nordmark NP (n=3 per group). Post-isolation, islets were assessed for purity (%), viability (%) and number (IEQ) then infused trans-hepatically via the portal vein. Statistical differences between the two experimental groups were determined by Mann Whitney Test. Results are presented as average ± standard deviation. Results: All 6 pancreases were severely fibrotic with calcification. Recipient age was statistically matched between the two protease groups (31±7 years and 30.6±10 years in the 100U and 200U NP groups respectively). Pancreas weight was also statistically comparable between the groups (100U group: 54.5±43g vs 200U group 75.6±33g). Islet purification was not performed for any of the 6 preparations. Although final islet yield was higher in the 100U group, this did not reach statistical significance (5,026.4±4,504 IEQ/g vs 3,947.8±2,696 IEQ/g). NP dose did not significantly impact on the final preparation purity (100U: 6.6±2.8% vs 200U: 11.6±7.8%) or viability (100U: 73.3±3.5% vs 200U: 71.6±5.6%). Final tissue volume was statistically comparable across the two groups (8±5.2ml vs 5.6±4.5ml in the 100U and 200U NP groups respectively). Conclusion: Our preliminary experience demonstrates the feasibility of using a decreased dose of protease when isolating islets from significantly fibrotic pancreases retrieved for autologous use. Using reduced protease would result in cost saving, and our data suggests it might reduce islet fragmentation and increase islet viability. However, enzyme selection must be considered carefully for each individual organ.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
ZZICU完成签到,获得积分10
1秒前
yun尘世发布了新的文献求助10
1秒前
量子星尘发布了新的文献求助10
2秒前
2秒前
4秒前
罗拉发布了新的文献求助10
4秒前
5秒前
5秒前
鱼e完成签到,获得积分10
7秒前
7秒前
8秒前
8秒前
song发布了新的文献求助10
10秒前
ding应助时尚初之采纳,获得10
10秒前
罗拉完成签到,获得积分10
10秒前
10秒前
11秒前
yun尘世完成签到,获得积分10
12秒前
12秒前
自信南霜完成签到,获得积分10
12秒前
tingting9完成签到,获得积分10
15秒前
15秒前
16秒前
卡布奇诺完成签到,获得积分10
16秒前
13223456发布了新的文献求助10
16秒前
青山落日秋月春风完成签到,获得积分10
18秒前
19秒前
20秒前
20秒前
小马甲应助动听的雅绿采纳,获得30
21秒前
1177发布了新的文献求助10
23秒前
23秒前
喜喵喵完成签到,获得积分10
24秒前
24秒前
24秒前
24秒前
11关注了科研通微信公众号
25秒前
123456完成签到,获得积分10
26秒前
时尚初之发布了新的文献求助10
26秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989242
求助须知:如何正确求助?哪些是违规求助? 3531393
关于积分的说明 11253753
捐赠科研通 3270010
什么是DOI,文献DOI怎么找? 1804868
邀请新用户注册赠送积分活动 882084
科研通“疑难数据库(出版商)”最低求助积分说明 809136