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Cellular Senescence Contributes to the Progression of Hyperoxic Bronchopulmonary Dysplasia

衰老 氧化应激 生物 DNA损伤 支气管肺发育不良 细胞生物学 炎症 癌症研究 免疫学 内分泌学 生物化学 遗传学 DNA 胎龄 怀孕
作者
Xigang Jing,Shuang Jia,Michelle H. Teng,Billy W. Day,Adeleye J. Afolayan,Jason A. Jarzembowski,Chien‐Wei Lin,Martin J. Hessner,Kirkwood A. Pritchard,Stephen Naylor,Girija G. Konduri,Ru‐Jeng Teng
出处
期刊:American Journal of Respiratory Cell and Molecular Biology [American Thoracic Society]
卷期号:70 (2): 94-109 被引量:9
标识
DOI:10.1165/rcmb.2023-0038oc
摘要

Oxidative stress (OS), inflammation, and endoplasmic reticulum (ER) stress sequentially occur in bronchopulmonary dysplasia (BPD), and all result in DNA damage. When DNA damage becomes irreparable, tumor suppressors increase, followed by apoptosis or senescence. Although cellular senescence contributes to wound healing, its persistence inhibits growth. Therefore, we hypothesized that cellular senescence contributes to BPD progression. Human autopsy lungs were obtained. Sprague-Dawley rat pups exposed to 95% oxygen between postnatal day 1 (P1) to P10 were used as the BPD phenotype. N-acetyl-lysyltyrosylcysteine-amide (KYC), tauroursodeoxycholic acid (TUDCA), and Foxo4dri were given i.p. to mitigate myeloperoxidase (MPO)-oxidant generation, ER stress, and cellular senescence, respectively. Lungs were examined by histology, transcriptomics, and immunoblots. Cellular senescence increased in rat and human BPD lungs, as evidenced by increased oxidative DNA damage, tumor suppressors, GL-13 stain, and inflammatory cytokines with decreased cell proliferation and lamin B expression. Cellular senescence-related transcripts in BPD rat lungs were enriched at P10 and P21. Single-cell RNA sequencing showed increased cellular senescence in several cell types, including type 2 alveolar cells (AT2). In addition, Foxo4-p53 binding increased in BPD rat lungs. Daily TUDCA or KYC, i.p., effectively decreased cellular senescence, improved alveolar complexity, and partially maintained the numbers of AT2. Foxo4dri given at P4, P6, P8, and P10 led to outcomes similar to TUDCA and KYC. Our data suggest that cellular senescence plays an essential role in BPD after initial inducement by hyperoxia. Reducing MPO toxic oxidant production, ER stress, and attenuating cellular senescence are potential therapeutic strategies for halting BPD progression.
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