离子色谱法
表征(材料科学)
色谱法
化学
离子交换
离子
纳米技术
材料科学
有机化学
作者
Akhilesh Kumar Kuril,Selvaraja Elumalai,Praveen Kumar Subbappa
出处
期刊:Current Analytical Chemistry
[Bentham Science]
日期:2024-10-01
卷期号:21
标识
DOI:10.2174/0115734110337889240920090945
摘要
Ion Exchange Chromatography (IEX) is an efficient and widely employed technique for the comprehensive characterization of therapeutic proteins and peptides. IEX separates biomolecules based on differences in their surface charge arising from amino acid composition and Post- Translational Modifications (PTMs). As a non-denaturing method, IEX is particularly valuable for resolving and quantifying charge variants like deamidation, oxidation, glycosylation, and terminal truncations in monoclonal antibodies, antibody-drug conjugates, and other biotherapeutics. The review provides insights into the principles, advantages, applications, recent advances, and limitations of using IEX (cation and anion exchange modes) for detailed analysis of proteins and peptides.
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