Molecular Biology and Genetic Engineering of Yeasts

Yeast Transformation. Yeast Plasmids: The 2 |m Plasmid of Saccharomyces cerevisiae. Yeast Plasmids: Linear and Circular Plasmids from Kluyveromyces. Transposable Elements in Yeast. Transcriptional Control of Gene Expression. Regulation of the Galactose Genes. Protein Localization and Processing in Yeast: Nuclear Targeting. Protein Localization and Processing in Yeast: Vacuolar Protein Targeting. Protein Localization and Processing in Yeast: Targeting Proteins into Mitochondria. Protein Localization and Processing in Yeast: Peroxisomes. Protein Localization and Processing in Yeast: Targeting of Integral Membrane Proteins. Protein Secretion. Saccharomyces cerevisiae Mating Pheromones. The Killer Toxin of Saccharomyces cerevisiae. Saccharomyces cerevisiae Invertase. Saccharomyces cerevisiae Acid Phosphatase. The Ubiquitin System and Its Role in the Genetic Control of Proteins Half-Life. Heterologous Expression and Secretion.