岩藻糖
软骨素
硫酸化
化学
硫酸软骨素
海参
单糖
多糖
生物化学
硫酸盐
糖胺聚糖
显著性差异
低聚糖
生物
糖蛋白
内科学
有机化学
生态学
医学
作者
Shiguo Chen,Changhu Xue,Yin Liang,Qingjuan Tang,Guangli Yu,Wengang Chai
标识
DOI:10.1016/j.carbpol.2010.08.040
摘要
Fucosylated chondroitin sulfates (fCSs) were isolated from four sea cucumbers Pearsonothuria graeffei (Indo-Pacific), Stichopus tremulus (Western Indian Ocean), Holothuria vagabunda (Norwegian coast), and Isostichopus badionotus (Western Atlantic). The detailed sequences of fCSs, particularly their fucose branches, were characterized and compared. 1H and 13C NMR of the polysaccharides clearly identified three different sulfation patterns on the branched fucoses, 4-O-mono-, 2,3-O-di- and 2,4-O-di-sulfation, variously distributed in the four fCSs. The chondroitin sulfate backbones were established based on the monosaccharide composition of the polysaccharides and two-dimensional NMR of their oligosaccharide fragments. Anticoagulant activities of the four fCSs were assessed and compared. The results indicated that the difference in their anticoagulant activities can be attributed to the difference in sulfation pattern of the fucose branch of the chondroitin sulfate, and 2,4-O-disulfation is important for anticoagulant activity.
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