Effect of Long-term Passive Smoking on Erectile Function and Penile Nitric Oxide Synthase in the Rat

No AccessJournal of UrologyInvestigative Urology1 Mar 1997Effect of Long-term Passive Smoking on Erectile Function and Penile Nitric Oxide Synthase in the Rat Yining Xie, Hermes Garban, Chris Ng, Jacob Rajfer, and Nestor F. Gonzalez-Cadavid Yining XieYining Xie , Hermes GarbanHermes Garban , Chris NgChris Ng , Jacob RajferJacob Rajfer , and Nestor F. Gonzalez-CadavidNestor F. Gonzalez-Cadavid View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)65153-XAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Given that smoking is a risk factor for erectile dysfunction, this study aimed to determine, in a rat model, whether long-term exposure to cigarette smoke impairs nitric oxide (NO)-dependent erectile function and reduces penile nitric oxide synthase (NOS), and if these changes are accompanied with effects on the systemic blood pressure. Materials and Methods: Adult (5 month) and old (20 month) rats were exposed to daily passive smoking for 8 wks. Three days after the conclusion of exposure, half of the animals were submitted to electrical field stimulation (EFS) of the cavernosal nerve and the maximum intracavernosal pressure (MIP) and mean arterial pressure (MAP) were determined and expressed as mm. Hg. On the other half of the animals, NOS activity in the penile cytosol was measured by the arginine/citrulline assay, and neuronal NOS (nNOS) and endothelial NOS (eNOS) contents were estimated by western blot and densitometry. Results: When compared to controls, the smoking rats had a higher MAP in both the adult (115 vs 162) and old (113 vs 140) rats, but surprisingly the MIP also increased, from 78 to 111 (adult rats) and from 59 to 83 (old rats). Smoking reduced penile NOS activity by 73% (adult rats), and 62% (old rats), and nNOS content by 43% and 50%, respectively. In contrast, eNOS was not affected. 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Google Scholar Department of Surgery, Division of Urology, UCLA School of Medicine, Harbor-UCLA Medical Center, Torrance, California© 1997 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byBivalacqua T, Sussan T, Gebska M, Strong T, Berkowitz D, Biswal S, Burnett A and Champion H (2018) Sildenafil Inhibits Superoxide Formation and Prevents Endothelial Dysfunction in a Mouse Model of Secondhand Smoke Induced Erectile DysfunctionJournal of Urology, VOL. 181, NO. 2, (899-906), Online publication date: 1-Feb-2009.McVARY K, CARRIER S and WESSELLS H (2018) SMOKING AND ERECTILE DYSFUNCTION: EVIDENCE BASED ANALYSISJournal of Urology, VOL. 166, NO. 5, (1624-1632), Online publication date: 1-Nov-2001.BIVALACQUA T, DINER E, NOVAK T, VOHRA Y, SIKKA S, CHAMPION H, KADOWITZ P and HELLSTROM W (2018) A RAT MODEL OF PEYRONIE'S DISEASE ASSOCIATED WITH A DECREASE IN ERECTILE ACTIVITY AND AN INCREASE IN INDUCIBLE NITRIC OXIDE SYNTHASE PROTEIN EXPRESSIONJournal of Urology, VOL. 163, NO. 6, (1992-1998), Online publication date: 1-Jun-2000.Moody J, Vernet D, Laidlaw S, Rajfer J and Gonzalez-Cadavid N (2018) Effects of Long-term Oral Administration of L-Arginine on the Rat Erectile ResponseJournal of Urology, VOL. 158, NO. 3, (942-947), Online publication date: 1-Sep-1997. Volume 157Issue 3March 1997Page: 1121-1126 Advertisement Copyright & Permissions© 1997 by American Urological Association, Inc.MetricsAuthor Information Yining Xie More articles by this author Hermes Garban More articles by this author Chris Ng More articles by this author Jacob Rajfer More articles by this author Nestor F. Gonzalez-Cadavid More articles by this author Expand All Advertisement PDF downloadLoading ...