化学
肉桂酸
活性氧
超氧化物歧化酶
过氧化氢
NAD+激酶
抗氧化剂
活力测定
西妥因1
细胞凋亡
乳酸脱氢酶
谷胱甘肽
氧化应激
生物化学
药理学
酶
下调和上调
生物
基因
作者
Yan Hu,Ning Zhang,Qi-Wen Fan,Musen Lin,Ce Zhang,Genlian Fan,Xuguang Zhai,Feng Zhang,Zhao Chen,Jihong Yao
标识
DOI:10.1139/cjpp-2014-0513
摘要
Carnosic acid (CA), found in rosemary, has been reported to have antioxidant and antiadipogenic properties. Here, we investigate the molecular mechanism by which CA inhibits hydrogen peroxide (H 2 O 2 )-induced injury in HepG2 cells. Cells were pretreated with 2.5–10 μmol/L CA for 2 h and then exposed to 3 mmol/L H 2 O 2 for an additional 4 h. CA dose-dependently increased cell viability and decreased lactate dehydrogenase activities. Pretreatment with CA completely attenuated the inhibited expression of manganese superoxide dismutase (MnSOD) and the B-cell lymphoma-extra large (Bcl-xL), and reduced glutathione activity caused by H 2 O 2 , whereas it reversed reactive oxygen species accumulation and the increase in cleaved caspase-3. Importantly, sirtuin 1 (SIRT1), a NAD + -dependent deacetylase, was significantly increased by CA. Considering the above results, we hypothesized that SIRT1 may play important roles in the protective effects of CA in injury induced by H 2 O 2 . As expected, SIRT1 suppression by Ex527 (6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide) and siRNA-mediated SIRT1 silencing (si-SIRT1) significantly aggravated the H 2 O 2 -induced increased level of cleaved caspase-3 but greatly reduced the decreased expression of MnSOD and Bcl-xL. Furthermore, the positive regulatory effect of CA was inhibited by si-SIRT1. Collectively, the present study indicated that CA can alleviate H 2 O 2 -induced hepatocyte damage through the SIRT1 pathway.
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